Imperial College London/Notebook/25 September 2009

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(New page: =Weekly Summary= ==What has been achieved==)
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=Weekly Summary=
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==Testing team==
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===OD calibration===
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==What has been achieved==
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*Carried out 10<sup>-4</sup> and 10<sup>-6</sup> dilutions
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*Carried out on ice
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*Used 3 OD values: 1, 0.7 and 0.4
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*Conclusions:
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**Too many colonies on 10<sup>-4</sup>, too few on 10<sup>-6</sup>, 10<sup>-5</sup> seems to be the most appropriate
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*Suggestions:
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**Dilute using factors: 10<sup>-4</sup>, 10<sup>-5</sup> and 10<sup>-6</sup> with 100uL inoculation

Revision as of 15:16, 1 October 2009

Testing team

OD calibration

  • Carried out 10-4 and 10-6 dilutions
  • Carried out on ice
  • Used 3 OD values: 1, 0.7 and 0.4
  • Conclusions:
    • Too many colonies on 10-4, too few on 10-6, 10-5 seems to be the most appropriate
  • Suggestions:
    • Dilute using factors: 10-4, 10-5 and 10-6 with 100uL inoculation
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