Team:UC Davis

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An estimated one in every 133 Americans suffer from an autoimmune disorder called celiac disease (14, 11). Celiac disease is a condition in which the small intestines are unable to digest gliadin, a protein present in gluten. When gliadin is consumed by an individual with celiac disease, the immune system responds by causing a range of side-effects, which may include abdominal pain, chronic diarrhea, and/or vomiting (14). A friend of one of our teammates currently suffers from this illness, which prompted us to look into celiac disease as the focus of our project. Currently, the only accepted treatment for this disorder is adhering to a gluten-free diet (14, 12). This inspired us to design a probiotic organism that could survive and take residence in the stomach, where it would secrete an enzyme that can degrade gliadin. This would prevent gliadin from progressing through the small intestine and inducing immune system response.
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This synthetically engineered organism should be able to satisfy two main criteria. First, it should be able to survive only in the stomach and secondly, it should secrete an enzyme that can degrade gliadin.  
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Studies have shown that the enzyme, Prolyl-endoprotease, is able to degrade gliadin at the pH level of the stomach (8). Prolyl-endoprotease is one of the most promising enzymes which are being used in recent studies regarding treatment for celiac disease.
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Due to licensing restrictions, we have opted not to work with this protein.
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<div style="text-align: center;"><span
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style="color: rgb(255, 255, 153);">&nbsp;
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We have decided to focus our summer project on building two key elements required for our desired organism:
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</span><b style="color: rgb(255, 255, 153);"><span
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# A secretion system
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# [https://2009.igem.org/Team:UC_Davis/pH_Sensor A biological pH sensor limiting this secretion system to the stomach]
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| [https://2009.igem.org/Team:UC_Davis Home] | [https://2009.igem.org/Team:UC_Davis/About_Us About Us] | [https://2009.igem.org/Team:UC_Davis/Celiac Celiac Disease] | [https://2009.igem.org/Team:UC_Davis/Treatments Current Treatment] | [https://2009.igem.org/Team:UC_Davis/Approach Our Approach] | [https://2009.igem.org/Team:UC_Davis/pH_Sensor pH Sensor] | [https://2009.igem.org/Team:UC_Davis/pH_Sensor/parts pH Sensor Parts] | [https://2009.igem.org/Team:UC_Davis/pH_Sensor/promoters pH Promoters] | [https://2009.igem.org/Team:UC_Davis/Secretion/parts Secretion Parts] |
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big>&nbsp;
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</big></big></big></big></span></b><b style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><a
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href="https://2009.igem.org/Team:UC_Davis/About_Us1"><img alt=""
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src="https://static.igem.org/mediawiki/2009/d/d1/UCDAVIS_PIC4.png"
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style="border: 0px solid ; width: 92px; height: 46px;"></a> </big></big></big></big></span></b><b
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><a
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href="https://2009.igem.org/Team:UC_Davis/Project1"><img alt=""
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src="https://static.igem.org/mediawiki/2009/b/b9/UCDAVIS_PIC8.png"
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style="border: 0px solid ; width: 92px; height: 46px;"></a> </big></big></big></big></span></b><b
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style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><a
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href="https://2009.igem.org/Team:UC_Davis/Notebook1"><img alt=""
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src="https://static.igem.org/mediawiki/2009/2/2f/UCDAVIS_PIC5.png"
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style="border: 0px solid ; width: 92px; height: 46px;"></a> </big></big></big></big></span></b><b
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><a
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href="https://2009.igem.org/Team:UC_Davis/Parts_1"><img alt=""
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src="https://static.igem.org/mediawiki/2009/a/a6/UCDAVIS_PIC6.png"
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style="border: 0px solid ; width: 92px; height: 46px;"></a>&nbsp;<a
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href="https://2009.igem.org/Team:UC_Davis/Contact1"><img alt=""
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src="https://static.igem.org/mediawiki/2009/1/1d/UCDAVIS_PIC7.png"
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style="border: 0px solid ; width: 92px; height: 46px;"></a></big></big></big></big></span></b><b
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style="color: rgb(0, 0, 0);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><small>&nbsp;
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&nbsp;<img style="width: 191px; height: 147px;" alt=""
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src="https://static.igem.org/mediawiki/2009/b/b8/UCDAVIS_PIC2.png">
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</small></big></big></big></big></span></b></div>
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<hr style="width: 100%; height: 2px;"><b style="color: rgb(0, 0, 0);"><span
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style="margin-bottom: 0.0001pt; line-height: normal;"><b><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;">&nbsp;&nbsp;&nbsp;
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&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;<big> </big></span></b><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black; font-weight: bold;"><big>&nbsp;
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</big><big>It
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is estimated that about one in
 +
every 133 Americans suffer from an autoimmune disorder called Celiac
 +
Disease
 +
(14, 11). Celiac Disease is a condition where the small intestines of
 +
an
 +
affected individual cannot digest gliadin, a protein present in gluten.
 +
When
 +
consumed, gliadin kick starts immune system responses, whose
 +
side-effects are,
 +
but not limited to abdominal pain, chronic diarrhea and vomiting (14).
 +
One of
 +
our teammate’s friends is currently suffering from this illness, which
 +
interested us to look into Celiac Disease as the focus of our project.
 +
Currently, the only accepted treatment for this disorder is adhering to
 +
a
 +
gluten-free diet (14, 12). This can be very difficult as great
 +
varieties of
 +
food contain gluten. This inspired us to design a probiotic organism
 +
that could
 +
survive and take residence in the stomach where it would secrete an
 +
enzyme that
 +
can degrade gliadin.</big></span><big style="font-weight: bold;"><span
 +
style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><br>
 +
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; &nbsp;&nbsp; This synthetically
 +
engineered bug should be able to satisfy at least two main criteria.
 +
First, it
 +
should be able to survive ONLY in the stomach and, secondly, secrete an
 +
enzyme
 +
that can degrade gliadin<i>.</i>&nbsp; </span><span
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style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span><br>
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</big><span style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big
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style="font-weight: bold;">&nbsp;&nbsp;&nbsp;
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&nbsp; &nbsp;
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&nbsp;&nbsp; We have
 +
decided to focus our summer project on building two key elements
 +
required for
 +
our “dream bug”. Therefore, our project has been split into two tasks:<o:p></o:p><br>
 +
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;
 +
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; 1. Produce an inducible secretion
 +
system<br>
 +
</big>
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<big style="font-weight: bold;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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2. Construct a biological pH sensor limiting this secretion system to
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the
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system</big><span style="font-weight: bold;"><br>
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</span></span></p>
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<hr style="width: 100%; height: 2px;">
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</ul>
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<div style="text-align: center;"><big> </big></div>
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<div style="text-align: center;">
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<div style="text-align: center;"></div>
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<div style="text-align: left;">
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<div style="text-align: center;"><big><span style="font-weight: bold;"><big>&nbsp;&nbsp;
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&nbsp;&nbsp;<small>
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UC
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Davis iGEM team is sponsors:</small></big></span></big><br>
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<big><span style="font-weight: bold;"></span></big></div>
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<div style="text-align: center;">&nbsp;&nbsp; &nbsp;&nbsp; <img
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src="https://static.igem.org/mediawiki/2009/8/88/UCDAVIS_Biomedical_engineering.jpg"
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alt="" style="width: 83px; height: 95px;"><img
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style="width: 83px; height: 94px;" alt=""
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src="https://static.igem.org/mediawiki/2009/4/43/UCDAVIS_college_of_engineering.png"><img
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src="https://static.igem.org/mediawiki/2009/6/6a/UCDAVIS_Accutek.jpg"><img
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src="https://static.igem.org/mediawiki/2009/6/6b/UCDAVIS_mrgen.gif"><img
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src="https://static.igem.org/mediawiki/2009/c/c6/UCDAVIS_genome_center.jpg"><br>
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<br>
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Revision as of 20:51, 25 September 2009

Homepage_UCdavis

        

              It is estimated that about one in every 133 Americans suffer from an autoimmune disorder called Celiac Disease (14, 11). Celiac Disease is a condition where the small intestines of an affected individual cannot digest gliadin, a protein present in gluten. When consumed, gliadin kick starts immune system responses, whose side-effects are, but not limited to abdominal pain, chronic diarrhea and vomiting (14). One of our teammate’s friends is currently suffering from this illness, which interested us to look into Celiac Disease as the focus of our project. Currently, the only accepted treatment for this disorder is adhering to a gluten-free diet (14, 12). This can be very difficult as great varieties of food contain gluten. This inspired us to design a probiotic organism that could survive and take residence in the stomach where it would secrete an enzyme that can degrade gliadin.
           This synthetically engineered bug should be able to satisfy at least two main criteria. First, it should be able to survive ONLY in the stomach and, secondly, secrete an enzyme that can degrade gliadin. 
           We have decided to focus our summer project on building two key elements required for our “dream bug”. Therefore, our project has been split into two tasks:
                1. Produce an inducible secretion system
                 2. Construct a biological pH sensor limiting this secretion system to the system

      UC Davis iGEM team is sponsors:
     

                                         

Retrieved from "http://2009.igem.org/Team:UC_Davis"