Team:Chiba/Project/Delay Switch
From 2009.igem.org
(→Exp #3' LuxR Mutants (One More Plan : Direct Screening for Delay Switcher)) |
(→Exp #3' LuxR Mutants (One More Plan : Direct Screening for Delay Switcher)) |
||
Line 117: | Line 117: | ||
:In this plan, we introduced the random mutation into the entire reading frame of LuxR using error-prone PCR. | :In this plan, we introduced the random mutation into the entire reading frame of LuxR using error-prone PCR. | ||
|} | |} | ||
+ | |||
+ | https://static.igem.org/mediawiki/2009/b/b7/%E5%9B%B31.png | ||
=== Results (2009) === | === Results (2009) === |
Revision as of 15:15, 27 September 2009
|
E.coli Time Manager Since 2008
Constructing A Delay Switch Since 2008In principle, there are three ways to delay the activation of chemical communications;
Inter-species communications! We decided to go for the strategy inspired by Japanese-classic experience; Whenever we speak to somebody in English, we often experience a certain delay in activating the communication. We though this is exactly what we pursued in. See Exp #4. Same applies to the reverse, too. When somebody speaks to us, we definitely need some time (sometime infinite) to get activated. This is all in spite that he/ she was loud and clear enough. The less affinity (perception) we have to English, the longer we need to activate them.See exp #5. Experiments (2008)For details, click each of the index titles
Results (2008)In conclusion, we tried (and are trying) to device a series of delay-switches by designing the "switching consortia". We got limited, by certain success in generating delay switches. Experiments (2009)For details, click the index titles
Results (2009) |