Team:UNICAMP-Brazil/Notebooks/September 23

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==''' ColiGuard '''==
==''' ColiGuard '''==
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====Electroelution verification====
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====F plasmid recircularization====
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*The samples from yesterday's electroelution were applied to a 0,8% agarose gel and submitted to 80 V during 30 minutes. A band of approximately 22 kb was formed, indicating that the F plasmid was succesfully purified!
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*The reaction was assembled according to protocol 10. The reaction tube was left O/N at 4°C.
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*Now that the F plasmid was obtained, the next step is its recircularization.
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''Gabriel''
''Gabriel''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 20:56, 26 September 2009

ColiGuard

F plasmid recircularization

  • The reaction was assembled according to protocol 10. The reaction tube was left O/N at 4°C.

Gabriel




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