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Team:Newcastle/Labwork/30 July 2009
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(New page: =Lab Work - 30/07/09= ==Introduction== In the previous lab session, a number of different things happened. The team digested the three sets of mini-preps originating from ''E. coli'' trans...) |
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In the previous lab session, a number of different things happened. The team digested the three sets of mini-preps originating from ''E. coli'' transformed with ''BBa_C0056'', ''BBa_B1002'' and ''BBa_R0077'' with ''EcoRI'' and ''PstI''; when run on agarose gel it could be seen that the correct DNA had been transformed!. Also in the previous lab session the team attempted a second transformation of ''JM109'' ''E. coli'' cells with BioBricks ''BBa_C0077'' and ''BBa_C0076''. And finally the team chose some cells to be frozen down for long term storage and grew those selected cultures up in LB solutions. | In the previous lab session, a number of different things happened. The team digested the three sets of mini-preps originating from ''E. coli'' transformed with ''BBa_C0056'', ''BBa_B1002'' and ''BBa_R0077'' with ''EcoRI'' and ''PstI''; when run on agarose gel it could be seen that the correct DNA had been transformed!. Also in the previous lab session the team attempted a second transformation of ''JM109'' ''E. coli'' cells with BioBricks ''BBa_C0077'' and ''BBa_C0076''. And finally the team chose some cells to be frozen down for long term storage and grew those selected cultures up in LB solutions. | ||
| - | Today's lab work will involve completing the freezing down bacteria procedure. It will also involve observing the LB + Kan plates for any ''BBa_C0077'' and ''BBa_C0076'' transformants; if there are colonies then mini-preps will be conducted and if the transformations have failed then a final attempt at transforming ''E. coli'' cells will commence. | + | Today's lab work will involve completing the freezing down bacteria procedure. It will also involve observing the LB + Kan plates for any ''BBa_C0077'' and ''BBa_C0076'' transformants; if there are colonies then mini-preps will be conducted and if the transformations have failed then a final attempt at transforming ''E. coli'' cells will commence. The team will also be given a lesson by PhD student Chris Birchall on how to pour LB agar plates the way in which Dr. Phil Aldridge pours plates. |
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==Practical Outline== | ==Practical Outline== | ||
==Observations== | ==Observations== | ||
Revision as of 18:27, 29 September 2009
Contents |
Lab Work - 30/07/09
Introduction
In the previous lab session, a number of different things happened. The team digested the three sets of mini-preps originating from E. coli transformed with BBa_C0056, BBa_B1002 and BBa_R0077 with EcoRI and PstI; when run on agarose gel it could be seen that the correct DNA had been transformed!. Also in the previous lab session the team attempted a second transformation of JM109 E. coli cells with BioBricks BBa_C0077 and BBa_C0076. And finally the team chose some cells to be frozen down for long term storage and grew those selected cultures up in LB solutions.
Today's lab work will involve completing the freezing down bacteria procedure. It will also involve observing the LB + Kan plates for any BBa_C0077 and BBa_C0076 transformants; if there are colonies then mini-preps will be conducted and if the transformations have failed then a final attempt at transforming E. coli cells will commence. The team will also be given a lesson by PhD student Chris Birchall on how to pour LB agar plates the way in which Dr. Phil Aldridge pours plates.
