Team:Utah State/Project

From 2009.igem.org

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Revision as of 18:57, 9 October 2009

USUlogo.jpg

Untitled Document


Welcome!

OUR SITE IS STILL UNDER CONSTRUCTION AND OUR INFORMATION IS BEING ADDED. PLEASE COME BACK IN A FEW WEEKS TO SEE OUR PROJECT!


This is where more text can go



Contents

Overall project

Bacteria without Borders: Investigating a multi-host BioBrick vector and secretion of cellular products

The aim of the Utah State University iGEM project is to develop improved upstream and downstream processing strategies for manufacturing cellular products using the standardized BioBrick system. First, we altered the broad-host range vector pRL1383a to comply with BioBrick standards and enable use of BioBrick constructs in organisms like Pseudomonas putida, Rhodobacter sphaeroides, and Synechocystis PCC6803. This vector will facilitate exploitation of advantageous characteristics of these organisms, such as photosynthetic carbon assimilation. Following expression, product recovery poses a difficult and expensive challenge. Downstream processing of cellular compounds, like polyhydroxyalkanoates (PHAs), commonly represents more than half of the total production expense. To counter this problem, secretion-promoting BioBrick devices were constructed through genetic fusion of signal peptides with protein-coding regions. To demonstrate this, the secretion of PHA granule-associated proteins and their affinity to PHA was investigated. Project success will facilitate expression and recovery of BioBrick-coded products in multiple organisms.

Project Details


Protocols

Part 2

The Experiments

Part 3

Results

Retrieved from "http://2009.igem.org/Team:Utah_State/Project"