Team:UC Davis/Adding secretion
From 2009.igem.org
(Difference between revisions)
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of having several models:</span></b><b><span | of having several models:</span></b><b><span | ||
style="font-size: 12pt; font-family: "Times New Roman","serif";"><o:p></o:p></span></b></p> | style="font-size: 12pt; font-family: "Times New Roman","serif";"><o:p></o:p></span></b></p> | ||
- | < | + | <div style="text-align: left;"> The |
- | + | purpose of the secretion system is to introduce a method | |
- | In | + | of excreting target proteins we wish to synthesize in the marvelous |
- | our secretion system, we are using two genes with different sizes as | + | host: E.coli |
- | our target | + | HB101. Through this approach of being able to secrete specific |
- | secretion genes, GFP being short in length and Luciferase being | + | target |
- | comparably long | + | proteins, we hope to serve as a chassis to the rest of the biological |
- | would test our secretion system and its ability to secrete small and | + | world.<br> |
- | large | + | In our secretion system, we are using |
- | proteins. <br> | + | two |
- | Testing multiple different combinations; | + | genes with different sizes as our target secretion genes, GFP being |
- | Signal | + | short in |
- | Sequence alone, Signal Sequence plus ompA/INPNC and ompA/INPNC alone, | + | length and Luciferase being comparably long would test our secretion |
- | would | + | system and |
- | help us find the best combination for our secretion system based on | + | its ability to secrete small and large proteins. <br> |
- | their | + | Testing multiple different |
- | secretion ability and rank them from strongest to weakest in strength | + | combinations; Signal Sequence alone, Signal Sequence plus ompA/INPNC |
- | (in this | + | and |
- | specific system).</ | + | ompA/INPNC alone, would help us find the best combination for our |
- | <big><big><big><span style="font-weight: bold;"><small><span | + | secretion |
+ | system based on their secretion ability and rank them from strongest to | ||
+ | weakest | ||
+ | in strength (in this specific system).</div> | ||
+ | <div style="text-align: left;"><big><big><big><span | ||
+ | style="font-weight: bold;"><small><span | ||
style="text-decoration: underline; background-color: rgb(255, 255, 0);"></span></small></span></big></big></big><br> | style="text-decoration: underline; background-color: rgb(255, 255, 0);"></span></small></span></big></big></big><br> | ||
+ | </div> | ||
<div style="text-align: left;"> | <div style="text-align: left;"> | ||
<hr style="width: 100%; height: 2px;"><big><small><big | <hr style="width: 100%; height: 2px;"><big><small><big | ||
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style="font-size: 14pt; font-family: "Times New Roman","serif";"><small><small>Number | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small><small>Number | ||
of polypeptide</small></small><o:p></o:p></span></b></small></p> | of polypeptide</small></small><o:p></o:p></span></b></small></p> | ||
- | + | </td> | |
</tr> | </tr> | ||
<tr style=""> | <tr style=""> | ||
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style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | ||
style="font-size: 14pt; font-family: "Times New Roman","serif";"><small><small>Cytochrome | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small><small>Cytochrome | ||
- | <i>c </i>(human</small></small></span></b></p> | + | <i>c </i>(human )<br> |
+ | </small></small></span></b></p> | ||
</td> | </td> | ||
<td | <td | ||
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<p class="MsoNormal" | <p class="MsoNormal" | ||
style="margin-bottom: 0.0001pt; line-height: normal;"><small><small><b><span | style="margin-bottom: 0.0001pt; line-height: normal;"><small><small><b><span | ||
- | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small>13,000</small | + | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small>13,000</small></span></b></small></small></p> |
<small><small> </small></small></td> | <small><small> </small></small></td> | ||
<td | <td | ||
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style="margin-bottom: 0.0001pt; line-height: normal;"><small><b><span | style="margin-bottom: 0.0001pt; line-height: normal;"><small><b><span | ||
style="font-size: 14pt; font-family: "Times New Roman","serif";"><small>104</small><o:p></o:p></span></b></small></p> | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small>104</small><o:p></o:p></span></b></small></p> | ||
- | + | </td> | |
<td | <td | ||
style="border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; border-width: medium 1pt 1pt medium; padding: 0in 5.4pt; width: 118.25pt; background-color: rgb(0, 0, 153); color: rgb(255, 255, 0);" | style="border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; border-width: medium 1pt 1pt medium; padding: 0in 5.4pt; width: 118.25pt; background-color: rgb(0, 0, 153); color: rgb(255, 255, 0);" | ||
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style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | ||
- | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small>1</small><o:p></o:p></span></b></p> | + | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small> <br> |
+ | 1</small><o:p></o:p></span></b></p> | ||
</td> | </td> | ||
</tr> | </tr> | ||
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style="border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; border-width: medium 1pt 1pt medium; padding: 0in 5.4pt; width: 117.5pt; height: 51.25pt; background-color: rgb(0, 0, 153); color: rgb(255, 255, 0);" | style="border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; border-width: medium 1pt 1pt medium; padding: 0in 5.4pt; width: 117.5pt; height: 51.25pt; background-color: rgb(0, 0, 153); color: rgb(255, 255, 0);" | ||
valign="top" width="157"> | valign="top" width="157"> | ||
+ | <p class="MsoNormal" | ||
+ | style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | ||
+ | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small><br> | ||
+ | </small></span></b></p> | ||
<p class="MsoNormal" | <p class="MsoNormal" | ||
style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | ||
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style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | ||
- | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small>1</small><o:p></o:p></span></b></p> | + | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small><br> |
+ | 1</small><o:p></o:p></span></b></p> | ||
</td> | </td> | ||
</tr> | </tr> | ||
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<p class="MsoNormal" | <p class="MsoNormal" | ||
style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | ||
- | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small>609</small><o:p></o:p></span></b></p> | + | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small><br> |
+ | 609</small><o:p></o:p></span></b></p> | ||
</td> | </td> | ||
<td | <td | ||
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<p class="MsoNormal" | <p class="MsoNormal" | ||
style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | style="margin-bottom: 0.0001pt; line-height: normal;"><b><span | ||
- | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small>1</small><o:p></o:p></span></b></p> | + | style="font-size: 14pt; font-family: "Times New Roman","serif";"><small> <br> |
+ | 1</small><o:p></o:p></span></b></p> | ||
</td> | </td> | ||
</tr> | </tr> |
Revision as of 03:19, 13 October 2009
Adding Secretion:
General
model for secretion system:
Secretion Models:
Click on a specific model for more information:
Advantages
of having several models:
The
purpose of the secretion system is to introduce a method
of excreting target proteins we wish to synthesize in the marvelous
host: E.coli
HB101. Through this approach of being able to secrete specific
target
proteins, we hope to serve as a chassis to the rest of the biological
world.
In our secretion system, we are using two genes with different sizes as our target secretion genes, GFP being short in length and Luciferase being comparably long would test our secretion system and its ability to secrete small and large proteins.
Testing multiple different combinations; Signal Sequence alone, Signal Sequence plus ompA/INPNC and ompA/INPNC alone, would help us find the best combination for our secretion system based on their secretion ability and rank them from strongest to weakest in strength (in this specific system).
In our secretion system, we are using two genes with different sizes as our target secretion genes, GFP being short in length and Luciferase being comparably long would test our secretion system and its ability to secrete small and large proteins.
Testing multiple different combinations; Signal Sequence alone, Signal Sequence plus ompA/INPNC and ompA/INPNC alone, would help us find the best combination for our secretion system based on their secretion ability and rank them from strongest to weakest in strength (in this specific system).
Secretion Models:
Click on a specific model for more information:
There
is wide range of genes present in our environment. Therefore, it is
important
to measure our secretion system’s ability to secrete genes of various
sizes.
Molecular data on some proteins
|
Molecular |
Number
of residues |
Number
of polypeptide |
Cytochrome
c (human ) |
13,000 |
104 |
|
Ribonuclease
A(bovine pancreas) |
|
124 |
|
Serum
albumin (human) |
|
|
|
(Page
128, third edition “Lehninger
Principles of Biochemistry” by David L. Nelson and Michael M. Cox)