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Team:UNICAMP-Brazil/Notebooks/September 30
From 2009.igem.org
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- This morning we found some ''E. coli'' colonies that were transformed yesterday! We performed PCR with 10 colonies from each biobrick (pJEN1, pDLD and Lysozyme) with the vector's primers (VR and VF). | - This morning we found some ''E. coli'' colonies that were transformed yesterday! We performed PCR with 10 colonies from each biobrick (pJEN1, pDLD and Lysozyme) with the vector's primers (VR and VF). | ||
- The figure shows that unfortunately none of the colonies tested had the insert into the vector. The size of the amplicons correspond to the vector's fragment without any insert (+/- 1400bp). | - The figure shows that unfortunately none of the colonies tested had the insert into the vector. The size of the amplicons correspond to the vector's fragment without any insert (+/- 1400bp). | ||
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| + | GEL | ||
- We also decided to try to do a miniprep using the rest of the transformed ''E. coli'' that was not plated in LB+AMP media. So today we made the inoculum. | - We also decided to try to do a miniprep using the rest of the transformed ''E. coli'' that was not plated in LB+AMP media. So today we made the inoculum. | ||
Revision as of 13:16, 2 October 2009
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