Team:UNICAMP-Brazil/Notebooks/October 1

From 2009.igem.org

(Difference between revisions)
(→JEN1 ORF)
(→Preparation of electrocompetent E. coli)
Line 8: Line 8:
====Preparation of electrocompetent ''E. coli''====
====Preparation of electrocompetent ''E. coli''====
-
* Today we prepared more electrocompetent ''E. coli'' to use in our transformations according to Protocol 4 (see protocols section).
+
* Today we prepared more electrocompetent ''E. coli'' to use in our transformations according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Preparation_of_electrocompetent_E._coli Protocol 4].
''Marcos and Taís''
''Marcos and Taís''

Revision as of 20:55, 3 October 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

MicroGuards

Preparation of electrocompetent E. coli

  • Today we prepared more electrocompetent E. coli to use in our transformations according to Protocol 4.

Marcos and Taís

YeastGuard

New biobricks - "screening" by miniprep and digestion

- Today we did the miniprep using the rest of the transformed E. coli that was not plated in LB+AMP media. After that we digested the miniprep with Xba1, in order to find the band with the correct molecular weight (expected for the inserts connected to the vector). Unfortunately we only found one band with the wrong molecular weight.

GEL


JEN1 ORF

- The concentration of the new part JEN1ORF wasn´t enough for the ligation reactions. So we repeated the PCR and today we digested it with Xba1 and Spe1.

Terminator Biobrick

- We digested the terminator briobrick with Xba1 and Pst1.

Taís





Retrieved from "http://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/October_1"