Team:UNICAMP-Brazil/Protocols

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'''[[Team:UNICAMP-Brazil/Protocols/T4 DNA Ligase|11. T4 DNA Ligase]]'''
'''[[Team:UNICAMP-Brazil/Protocols/T4 DNA Ligase|11. T4 DNA Ligase]]'''
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'''[[Team:UNICAMP-Brazil/Protocols/Electroelution|12. Electroelution]]'''
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Revision as of 14:11, 2 October 2009

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Protocols

1. Genomic DNA Extraction

2. Mini-Prep

3. Transformation

4. Preparation of electrocompetent E. coli

5. Yeast DNA Extraction

6. Yeast RNA Extraction

7. Purification of DNA fragments from agarose gels

8. Isolation of RNA using Trizol Reagent (Invitrogen)

9. SAP Dephosphorylation

10. CIAP Dephosphorylation

11. T4 DNA Ligase

12. Electroelution