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| - | ==Testing==
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| - | ===Diauxic growth 11th Sep===
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| - | ====Protocol considerations====
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| - | *Using spectrophotometer at 600nm
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| - | *Blanks are all the relevant carbon sources, but subsequently realised that all blanks were roughly the same (within 0.03)
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| - | **Therefore, subsequent readings= blank + 6 sample
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| - | *Overnight culture had OD of 1.64.
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| - | **800ul was put into each sample of 40ml~ 0.033 OD
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| - | **ie same initial OD
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| - | *Grown in 6 separate 50ml falcon tubes and shaking in incubator at 28 degrees Celsius
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| - | *1ml taken out at 1 hr intervals from the falcon tube and placed into spectrophotometer
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| - | ====Results====
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| - | [[Image:Diauxic growth.jpg]]<br>
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| - | [[Media:Diauxic growth_11-09.xls| Download raw data]]
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| - | ====Conclusions====
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| - | *Glucose, as expected, had the highest growth rate
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| - | *Cells under all secondary carbon source conditions appear to stop growth at 6 hrs.
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| - | ** Therefore, we can suggest that the switch point is after 6 hours
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| - | **However, further data at longer time points are required to confirm this.
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| - | ====Suggestions/Improvements====
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| - | * Longer duration of taking readings (but currently limited to 8hrs- length of a day and multiplate reader evaporation)
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| - | * Earlier switch point can be induced by adding a smaller concentration of glucose to media
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| - | **suggested 0.01% Glucose for next experiment (we predict it will take around 2 hours)
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