Team:Tsinghua/Experiment1

From 2009.igem.org

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(Bottom-Up Approach)
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==Top-Down Approach==
==Top-Down Approach==
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==Synthesis of the Targeted Biobrick==
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=GenSniper Production Evaluation=
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==Production of the targeted gene therapy vector==
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==Functioning of the targeted gene therapy vector==
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Revision as of 05:50, 3 October 2009

Contents

Synthesis of the Gene Therapy Production System

Bottom-Up Approach

In the bottom-up approach, we amplify the biobricks from both the bacteriophage lambda and the adenovirus genome and incorporate them with a given order into molecular cloning vector(s).

Specifically, we choose two molecular cloning vectors to encode two sections of the gene therapy vector genome, pET28a and pACYCDuet1.

pET28a pACYCDuet1

Top-Down Approach

GenSniper Production Evaluation