Team:Tsinghua/Experiment1

From 2009.igem.org

(Difference between revisions)
(Bottom-Up Approach)
Line 5: Line 5:
Specifically, we choose two molecular cloning vectors to encode two sections of the gene therapy vector genome, pET28a and pACYCDuet1.
Specifically, we choose two molecular cloning vectors to encode two sections of the gene therapy vector genome, pET28a and pACYCDuet1.
-
[[Image:pET28a.jpg|400px|pET28a]]
+
[[Image:pET28a.jpg|450px|pET28a]]
-
[[Image:pACYCDuet1.jpg|400px|pACYCDuet1]]
+
[[Image:pACYCDuet1.jpg|450px|pACYCDuet1]]
==Top-Down Approach==
==Top-Down Approach==
=GenSniper Production Evaluation=
=GenSniper Production Evaluation=

Revision as of 05:50, 3 October 2009

Contents

Synthesis of the Gene Therapy Production System

Bottom-Up Approach

In the bottom-up approach, we amplify the biobricks from both the bacteriophage lambda and the adenovirus genome and incorporate them with a given order into molecular cloning vector(s).

Specifically, we choose two molecular cloning vectors to encode two sections of the gene therapy vector genome, pET28a and pACYCDuet1.

pET28a pACYCDuet1

Top-Down Approach

GenSniper Production Evaluation