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Team:Heidelberg/k4l1um
From 2009.igem.org
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| - | * [[n4tr1um]]Miniprep of GFP template plasmid, pcDNA5/FRT | + | * [[Team:HEIDELBERG/n4tr1um# Isolation of plasmid DNA by alkaline lysis (mini and maxiprep)]]Miniprep of GFP template plasmid, pcDNA5/FRT |
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Revision as of 13:37, 17 June 2009
6-15-2009
Lab: LV, SH, CZ
SH:
- Team:HEIDELBERG/n4tr1um# Isolation of plasmid DNA by alkaline lysis (mini and maxiprep)Miniprep of GFP template plasmid, pcDNA5/FRT
| Nr | pcDNA5/FRT | GFP |
|---|---|---|
| 1 | 4,7 | 39,7 |
| 2 | 5,9 | 14,9 |
| 3 | 3,7 | 3,8 |
| 4 | 5,0 | 7,8 |
- Maxiprep pcDNA/FRT 188.7ng/µL
LV:
- Extraction of CMV promoter from 2008 distribution
- Transformation of DH5a cell with CMV promoter
- portzughtr
6-16-2009
Lab: LV, SH, CZ
- Annealing of oligos (JeT, cFos, Min)
- Purification of cDNA via 2% / 3% agarosegel
- Site directed mutagenesis of pcDNA5/FRT
- Dpn1 digestion
- Transformation of DH5a
