Team:Washington/Accomplishments
From 2009.igem.org
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- Make more universal, to work with all proteins | - Make more universal, to work with all proteins | ||
+ | |||
- Optimize secretion-tag and nano-tag for fusion proteins | - Optimize secretion-tag and nano-tag for fusion proteins | ||
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- Optimize RBS's | - Optimize RBS's | ||
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- Move secretion sequence into new plasmid, not TetR | - Move secretion sequence into new plasmid, not TetR | ||
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- Try variety of cell lines. | - Try variety of cell lines. | ||
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- Use new CDS display vector to display streptavidin | - Use new CDS display vector to display streptavidin | ||
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- Design monomeric protein to bind peptide sequence | - Design monomeric protein to bind peptide sequence | ||
+ | |||
- Utilize other proteins on CDS to test for activity of other proteins | - Utilize other proteins on CDS to test for activity of other proteins | ||
Revision as of 17:11, 6 October 2009
Home | The Team | Project Description | Notebook | User Guide | Goals and Accomplishments |
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BBa_K215000
BBa_K215001
BBa_K215002
BBa_K215010
BBa_K215011
BBa_K215090
BBa_K215091
Current Status: Complete
Future directions:
- Make more universal, to work with all proteins
- Optimize secretion-tag and nano-tag for fusion proteins
BBa_K215100
BBa_K215101
BBa_K215102
BBa_K215103
BBa_K215104
BBa_K215105
BBa_K215106
BBa_K215107
BBa_K215108
BBa_K215109
Current Status: In progress
Future directions:
- Optimize RBS's
- Move secretion sequence into new plasmid, not TetR
- Try variety of cell lines.
BBa_K215200
BBa_K215201
BBa_K215210
BBa_K215211
BBa_K215250
BBa_K215251
BBa_K215260
BBa_K215261
Current Status: In progress
Future Directions:
- Use new CDS display vector to display streptavidin
- Design monomeric protein to bind peptide sequence
- Utilize other proteins on CDS to test for activity of other proteins
Biobricks Submitted | Current Status Report | Future Directions |
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Quick overview of what was done and the status of everyting. Maybe 3 paragraphs (display, secretion, conclusions)