Team:Freiburg bioware/cloning

From 2009.igem.org

(Difference between revisions)

Revision as of 19:19, 8 October 2009

FREiGEM

Parts

His+FluA+Split+Foki_pMA

His+FluA+SplitLinker+Foki in pMA Vector

nucleotid sequence of ORF-1:
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA GTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCT GCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGA TCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAG TTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCG TAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCG TTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGT CAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAA ACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCG AGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCC CAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAG TGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGA CTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACC GGTTAA

AA sequence of ORF-1:

MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ
DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG
QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN
SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

complete nucleotid sequence:

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@@ Line 134: / Line 134: @@
 <h4>AA sequence of ORF-1:<br></h4>
 <table border="0">
-<td align="left">
+<p align="justify"><td align="left">
-MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
+MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP<br>
-EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
+EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY<br>
-IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
+IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA<br>
-CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ
+CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ<br>
-DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG
+DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG<br>
-QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN
+QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN<br>
-SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG<br><br></td></table>
+SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG<br><br></p></td></table>
 <h4>complete nucleotid sequence:<br></h4>