Team:Alberta/ByteCreation

From 2009.igem.org

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     <h1>Standard Plasmids</h1>
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We believe that sharing one’s research with the community is an important responsibility, both to keep research accountable to the public and to open doors for the next generation to get involved. Even for those students who don’t pursue science, an exposure to what synthetic biology is allows them to make more informed, responsible choices as consumers and voters. Thus, through high school outreach, we’re setting a good example of good example of corporate social responsibility. </P>
 
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Finally, we strive to learn how to better communicate synthetic biology to students. In order to evaluate the impact of our outreach, we collect feedback forms after presentations and have an online survey.
 
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<P>In order to produce the specific sticky ends of each gene to be assembled, unique plasmids were developed and named pAB and pBA.  These plasmids were designed and constructed from pUC19 and contain the pMB1 high copy origin.  Unique cassettes were designed containing PstI, XbaI, and primer annealing regions complementary to the ‘A’ and ‘B’ ends. The cassette was synthesized and inserted using two restriction sites (EcoRI and NsiI).  This left an EcoRI site on the final plasmid as well a PstI scar site. The primer annealing regions in pAB are reverse to that in pBA so that compatible sticky ends can be produced in either plasmid.  Genes can be inserted using XbaI and PstI (or an enzyme which produces a compatible sticky end). The plasmids were originally designed for a previous but now obsolete system, where the sticky ends were generated via nicking enzymes.</p>
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Our Human Practices component consists of presentations and pamphlets for high school and junior high school students about synthetic biology, iGEM, and our project. We’re doing this outreach primarily for the high school students’ own interest and benefit. Synthetic biology is rapidly changing the biotechnology industry, and an understanding of synthetic biology would enrich a student’s consideration of career choices. Becoming excited about a potential career option can also provide motivation for academic success. Our outreach is also a service to the research community, as the more students who know about synthetic biology, the more who may pursue it as a career. A greater pool of human resources can increase the quantity and quality of research </P>
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We believe that sharing one’s research with the community is an important responsibility, both to keep research accountable to the public and to open doors for the next generation to get involved. Even for those students who don’t pursue science, an exposure to what synthetic biology is allows them to make more informed, responsible choices as consumers and voters. Thus, through high school outreach, we’re setting a good example of good example of corporate social responsibility. </P>
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Finally, we strive to learn how to better communicate synthetic biology to students. In order to evaluate the impact of our outreach, we collect feedback forms after presentations and have an online survey.  
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Revision as of 22:16, 17 October 2009

University of Alberta - BioBytes










































































































Standard Plasmids

In order to produce the specific sticky ends of each gene to be assembled, unique plasmids were developed and named pAB and pBA. These plasmids were designed and constructed from pUC19 and contain the pMB1 high copy origin. Unique cassettes were designed containing PstI, XbaI, and primer annealing regions complementary to the ‘A’ and ‘B’ ends. The cassette was synthesized and inserted using two restriction sites (EcoRI and NsiI). This left an EcoRI site on the final plasmid as well a PstI scar site. The primer annealing regions in pAB are reverse to that in pBA so that compatible sticky ends can be produced in either plasmid. Genes can be inserted using XbaI and PstI (or an enzyme which produces a compatible sticky end). The plasmids were originally designed for a previous but now obsolete system, where the sticky ends were generated via nicking enzymes.

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