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Team:Illinois/Research
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*[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1807950 Translational Control and Target Recognition by Escherichia coli Small RNAs in Vivo]: Johannes H. Urban and Jörg Vogel, 2007 | *[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1807950 Translational Control and Target Recognition by Escherichia coli Small RNAs in Vivo]: Johannes H. Urban and Jörg Vogel, 2007 | ||
Documents successful fusions of sRNA target sequences to GFP in order to study regulatory activity of various sRNAs. | Documents successful fusions of sRNA target sequences to GFP in order to study regulatory activity of various sRNAs. | ||
| + | |||
| + | *[http://nar.oxfordjournals.org/cgi/content/abstract/33/3/1040 Detection of 5'- and 3'-UTR-derived small RNAs and cis-encoded antisense RNAs in Escherichia coli]: Mitsuoki Kawano, April A. Reynolds, Juan Miranda-Rios, and Gisela Storz, 2005 | ||
| + | Describes sRNAs that act on the 3' untranslated region (UTR) of a target mRNA. | ||
== '''Protocols''' == | == '''Protocols''' == | ||
Revision as of 16:54, 26 June 2009
Contents |
Research
This page lists various sources used in our research organized by topic. Please note that most of the links to articles on this page will take you only to the abstract of the paper.
General
- [http://www.neb.com/nebecomm/default.asp New England Biolabs Web Page]
Includes catalog for restriction endonucleases/other lab products, a resource called [http://tools.neb.com/NEBcutter2/index.php NEBcutter] that tells you what restriction endonucleases will cut your gene/DNA sequence of interest, and a tool called the [http://www.neb.com/nebecomm/DoubleDigestCalculator.asp? Double Digest Finder] that will tell you how compatible two restriction endonucleases are by comparing what conditions they work best in.
- [http://genolist.pasteur.fr/Colibri/ Colibri]
Contains a complete list of genes in E. coli and their base pair sequences.
Small RNAs
- [http://arjournals.annualreviews.org/doi/abs/10.1146/annurev.micro.58.030603.123841?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dncbi.nlm.nih.gov The Small RNA Regulators of E. coli: Roles and Mechanisms]: Susan Gottesman, 2004
An overview on small RNAs and their functions, discussing the roles of Hfq and RNase E, and mentioning advantages to sRNA use.
- [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSN-4VNHRSC-B&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=997a0feec59ee5290a51b6ffcde5b7bb Regulatory RNAs in Bacteria]: Lauren S. Waters and Gisela Storz, Cell, Vol. 136, Issue 4, pg. 615-628, 2009
Discusses various RNA regulators in bacteria, including riboswitches and sRNAs. Gives examples of well-characterized sRNAs and lists advantages of sRNAs over other types of gene regulation.
- [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1807950 Translational Control and Target Recognition by Escherichia coli Small RNAs in Vivo]: Johannes H. Urban and Jörg Vogel, 2007
Documents successful fusions of sRNA target sequences to GFP in order to study regulatory activity of various sRNAs.
- [http://nar.oxfordjournals.org/cgi/content/abstract/33/3/1040 Detection of 5'- and 3'-UTR-derived small RNAs and cis-encoded antisense RNAs in Escherichia coli]: Mitsuoki Kawano, April A. Reynolds, Juan Miranda-Rios, and Gisela Storz, 2005
Describes sRNAs that act on the 3' untranslated region (UTR) of a target mRNA.
Protocols
- [http://www.protocol-online.org/ Protocol Online]
Useful website for finding protocols online.
- [http://www.5prime.com/media/29661/perfectprep%20spin%20mini%20kit%20manual_5prime_1048299_072007.pdf PerfectPrep Spin Mini Kit Manual]
Protocol for plasmid purification using the PerfectPrep Spin Mini Kit.
- Supplementary Material: Translational Control and Target Recognition by Escherichia coli Small RNAs in Vivo: Johannes H. Urban and Jörg Vogel, 2007
Includes step-by-step protocols for fusion cloning into a standard plasmid (pXG-10), fusion cloning via 5' RACE (pXG-20), and operon fusion cloning into pXG-30.
- [http://www.springerlink.com.proxy2.library.uiuc.edu/content/h752r3415261357p/ A Green Fluorescent Protein (GFP)-Based Plasmid System to Study Post-Transcriptional Control of Gene Expression In Vivo]: Johannes H. Urban and Jörg Vogel, 2009
Details a modular two-plasmid system that uses GFP to study sRNA regulation in vivo.
Questions about our Wiki page? Please email Dave Korenchan at korench1@illinois.edu.
