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Team:Waterloo/Project
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== '''Chromobricks: A Platform for Chromosome Engineering with BioBricks ''' == | == '''Chromobricks: A Platform for Chromosome Engineering with BioBricks ''' == | ||
| - | The aim of our project is to develop a fully-featured platform for chromosome engineering, allowing the in vivo assembly of a synthetic chromosome from interchangeable parts, followed by selective degradation of the native chromosome. We have designed a proof-of-concept for chromosome-building that will use the site-specific integrase of phage ΦC31 to integrate a BioBrick into a defined locus of the <i>E. coli</i> genome. Six pairs of integrase-targeted <i>att</i> sites have been designed to be non-cross-reactive in order to support repeatable cassette-exchange reactions for chromosome building. We have also written software to model the integrase-mediated rearrangement of DNA molecules containing <i>att</i> sites, to aid the design of more elaborate chromosome-building systems. To selectively degrade the native chromosome we designed a nuclease-based, inducible genome-degradation system. In its simplest form, our system can be used to integrate biological devices into a chromosome in situations requiring stable copy number and selection-free maintenance. | + | The aim of our project is to develop a fully-featured platform for chromosome engineering, allowing the in vivo <br/> |
| + | assembly of a synthetic chromosome from interchangeable parts, followed by selective degradation of the native <br/> | ||
| + | chromosome. We have designed a proof-of-concept for chromosome-building that will use the site-specific integrase of <br/> | ||
| + | phage ΦC31 to integrate a BioBrick into a defined locus of the <i>E. coli</i> genome. Six pairs of integrase-targeted<br/> | ||
| + | <i>att</i> sites have been designed to be non-cross-reactive in order to support repeatable cassette-exchange <br/> | ||
| + | reactions for chromosome building. We have also written software to model the integrase-mediated rearrangement of DNA <br/> | ||
| + | molecules containing <i>att</i> sites, to aid the design of more elaborate chromosome-building systems. To selectively<br/> | ||
| + | degrade the native chromosome we designed a nuclease-based, inducible genome-degradation system. In its simplest form,<br/> | ||
| + | our system can be used to integrate biological devices into a chromosome in situations requiring stable copy number<br/> | ||
| + | and selection-free maintenance. | ||
== Project Details== | == Project Details== | ||
Revision as of 03:34, 11 October 2009
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Chromobricks: A Platform for Chromosome Engineering with BioBricks
The aim of our project is to develop a fully-featured platform for chromosome engineering, allowing the in vivo
assembly of a synthetic chromosome from interchangeable parts, followed by selective degradation of the native
chromosome. We have designed a proof-of-concept for chromosome-building that will use the site-specific integrase of
phage ΦC31 to integrate a BioBrick into a defined locus of the E. coli genome. Six pairs of integrase-targeted
att sites have been designed to be non-cross-reactive in order to support repeatable cassette-exchange
reactions for chromosome building. We have also written software to model the integrase-mediated rearrangement of DNA
molecules containing att sites, to aid the design of more elaborate chromosome-building systems. To selectively
degrade the native chromosome we designed a nuclease-based, inducible genome-degradation system. In its simplest form,
our system can be used to integrate biological devices into a chromosome in situations requiring stable copy number
and selection-free maintenance.
