Team:Freiburg bioware/cloning

From 2009.igem.org

(Difference between revisions)
Line 356: Line 356:
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;HisFluA+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
+
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
</div>
</div>
<div class="art-PostContent">
<div class="art-PostContent">
Line 365: Line 365:
<tr>
<tr>
<td align="center">
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2009/thumb/a/ad/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bmiddellinker%2BFokA_pMA.jpg" name="HisFluA+middellinker+FokA in pMA Vector" width="480" height="480" />
+
<img src="https://static.igem.org/mediawiki/2009/thumb/a/ad/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bmiddellinker%2BFokA_pMA.jpg" name="His+FluA+middellinker+FokA in pMA Vector" width="480" height="480" />
-
<br><p align="center">HisFluA+middellinker+FokA in pMA Vector</p>
+
<br><p align="center">His+FluA+middellinker+FokA in pMA Vector</p>
</td>
</td>
<td align="left">
<td align="left">
Line 417: Line 417:
<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
-
<li> <a href="https://static.igem.org/mediawiki/2009/7/78/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFokA_pMA.txt">HisFluA+middellinker+FokA_pMA.txt</a></li>
+
<li> <a href="https://static.igem.org/mediawiki/2009/7/78/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFokA_pMA.txt">His+FluA+middellinker+FokA_pMA.txt</a></li>
<br>
<br>
<br>
<br>
<br>
<br>
 +
 +
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;HisFluA+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;">
 +
</div><a name="4"></a>
 +
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/c/ce/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.jpg" name="HisFluA+middellinker+Foki in pMA Vector" width="480" height="480" />
 +
<br><p align="center">HisFluA+middellinker+Foki in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 +
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 +
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 +
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
 +
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
 +
CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
 +
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
 +
TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
 +
CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
 +
ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 +
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 +
ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
 +
GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG
 +
AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT
 +
CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA
 +
TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT
 +
CTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCC
 +
GATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATA
 +
ATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAAT
 +
CAGACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCC
 +
GTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAG
 +
GCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAAT
 +
GGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAA
 +
AGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCG
 +
AGATCAATTTTACCGGTTAA
 +
 +
 +
 +
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
 +
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
 +
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 +
NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF
 +
MKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEEN
 +
QTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELL
 +
IGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 +
 +
 +
<br><br></p></td></table>
 +
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/9/92/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.txt">HisFluA+middellinker+Foki_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 +

Revision as of 19:44, 11 October 2009

FREiGEM

overview


His+FluA+Split+Foki_pMA
His+Dig+Split+FokA_pMA
DsbA+His+Dig+Split+FokA_pMA

 Parts

 His+FluA+Split+Foki_pMA


His+FluA+SplitLinker+Foki in pMA Vector

 nucleotid sequence of ORF-1:
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA GTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCT GCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGA TCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAG TTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCG TAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCG TTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGT CAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAA ACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCG AGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCC CAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAG TGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGA CTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACC GGTTAA

AA sequence of ORF-1:

MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ
DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG
QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN
SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

Commented GenBank file

  • His+FluA+Split+Foki_pMA.txt



    •  His+Dig+Split+FokA_pMA


    His+Dig+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACCAGCC TGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAA ATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATG TGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAA GACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGG CTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTT ATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCC TATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCG TTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAAT GGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTG AGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCA TAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTG GCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGT AAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKA YSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA QLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+Split+FokA_pMA.txt



    •  DsbA+His+Dig+Split+FokA_pMA


    DsbA+His+Dig+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC ATCGGCGACCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACG ACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAG TACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAA GTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGA AGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGC ACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTA CACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCG ACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAG AAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGAC CGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGG TGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAA GTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACC AGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCG GCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAA TATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTAC CCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCT ATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCC ATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAA AGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGC AGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAAC GAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTT CGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGA ACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTG ATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCG CCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MKKIWLALAGLVLAFSASATGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAA YPDHITKYGKCGWAEYTPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIG GVTQVGVSNVLSTDNKNYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIG SPVVDSQKLVYSDFSEAACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEE KKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGA IYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSV TEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKF NNGEINFTG

    Commented GenBank file

  • DsbA+His+Dig+Split+FokA_pMA.txt



    •  Strep+FluA+ShortLinker+FokI_pMA


    Strep+FluA+ShortLinker+FokI in pMA Vector

    		 nucleotid sequence of ORF-1:
    GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAA AAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGA ACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGA AAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTG GGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGAT CGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATC TGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAG ACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTC AAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCA ACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGG GCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGC AGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGA TCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    GWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFM KVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQ TRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLI GGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+ShortLinker+FokI_pMA.txt



    •  His+FluA+middellinker+FokA_pMA


    His+FluA+middellinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT CTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCC GATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATA ACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAAC CAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCC GTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAG GCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAAT GGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAA AGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGG AGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF MKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKEN QTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELL IGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+middellinker+FokA_pMA.txt



    •  HisFluA+middellinker+Foki_pMA


    HisFluA+middellinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT CTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCC GATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATA ATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAAT CAGACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCC GTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAG GCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAAT GGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAA AGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCG AGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF MKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEEN QTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELL IGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • HisFluA+middellinker+Foki_pMA.txt





    • PostHeaderIcon Blank

    text here


    PostHeaderIcon Blank



    contact:  freigem09@googlemail.com

    Retrieved from "http://2009.igem.org/Team:Freiburg_bioware/cloning"