Team:Freiburg bioware/cloning

From 2009.igem.org

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<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;HisFluA+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
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<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
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<div class="art-PostContent">
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<tr>
<tr>
<td align="center">
<td align="center">
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<img src="https://static.igem.org/mediawiki/2009/thumb/c/ce/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.jpg" name="HisFluA+middellinker+Foki in pMA Vector" width="480" height="480" />
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<img src="https://static.igem.org/mediawiki/2009/thumb/c/ce/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.jpg" name="His+FluA+middellinker+Foki in pMA Vector" width="480" height="480" />
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<br><p align="center">HisFluA+middellinker+Foki in pMA Vector</p>
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<br><p align="center">His+FluA+middellinker+Foki in pMA Vector</p>
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<td align="left">
<td align="left">
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<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
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<li> <a href="https://static.igem.org/mediawiki/2009/9/92/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.txt">HisFluA+middellinker+Foki_pMA.txt</a></li>
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<li> <a href="https://static.igem.org/mediawiki/2009/9/92/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.txt">His+FluA+middellinker+Foki_pMA.txt</a></li>
<br>
<br>
<br>
<br>
<br>
<br>
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<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+shortlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
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</div>
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<div class="art-PostContent">
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<div style="text-align: center;">
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</div><a name="4"></a>
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<table border="5">
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<tr>
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<td align="center">
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<img src="https://static.igem.org/mediawiki/2009/thumb/d/d4/Freiburg09_FluA%2BHis%2Bshortlinker%2BFokA_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bshortlinker%2BFokA_pMA.jpg" name="His+FluA+shortlinker+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">His+FluA+shortlinker+FokA in pMA Vector</p>
 +
</td>
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<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 +
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 +
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 +
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
 +
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
 +
CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
 +
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
 +
TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
 +
CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
 +
ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 +
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 +
ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
 +
GCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAG
 +
CTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGA
 +
GATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGG
 +
AGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGC
 +
CGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGG
 +
CGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTG
 +
GTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAAC
 +
AAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTAC
 +
AGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAG
 +
CACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTG
 +
TCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCT
 +
GACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTA
 +
CCGGTTAA
 +
 +
 +
 +
 +
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
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<p align="justify"><td align="left">
 +
HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
 +
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
 +
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 +
NNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVY
 +
GYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRN
 +
KHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGE
 +
MIKAGTLTLEEVRRKFNNGEINFTG
 +
 +
 +
 +
 +
<br><br></p></td></table>
 +
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/4/46/Freiburg09_FluA%2BHis%2Bshortlinker%2BFokA_pMA.txt">His+FluA+shortlinker+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>

Revision as of 19:50, 11 October 2009

FREiGEM

overview


His+FluA+Split+Foki_pMA
His+Dig+Split+FokA_pMA
DsbA+His+Dig+Split+FokA_pMA

 Parts

 His+FluA+Split+Foki_pMA


His+FluA+SplitLinker+Foki in pMA Vector

 nucleotid sequence of ORF-1:
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA GTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCT GCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGA TCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAG TTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCG TAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCG TTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGT CAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAA ACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCG AGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCC CAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAG TGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGA CTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACC GGTTAA

AA sequence of ORF-1:

MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ
DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG
QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN
SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

Commented GenBank file

  • His+FluA+Split+Foki_pMA.txt



    •  His+Dig+Split+FokA_pMA


    His+Dig+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACCAGCC TGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAA ATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATG TGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAA GACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGG CTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTT ATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCC TATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCG TTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAAT GGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTG AGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCA TAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTG GCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGT AAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKA YSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA QLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+Split+FokA_pMA.txt



    •  DsbA+His+Dig+Split+FokA_pMA


    DsbA+His+Dig+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC ATCGGCGACCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACG ACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAG TACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAA GTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGA AGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGC ACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTA CACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCG ACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAG AAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGAC CGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGG TGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAA GTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACC AGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCG GCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAA TATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTAC CCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCT ATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCC ATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAA AGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGC AGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAAC GAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTT CGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGA ACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTG ATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCG CCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MKKIWLALAGLVLAFSASATGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAA YPDHITKYGKCGWAEYTPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIG GVTQVGVSNVLSTDNKNYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIG SPVVDSQKLVYSDFSEAACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEE KKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGA IYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSV TEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKF NNGEINFTG

    Commented GenBank file

  • DsbA+His+Dig+Split+FokA_pMA.txt



    •  Strep+FluA+ShortLinker+FokI_pMA


    Strep+FluA+ShortLinker+FokI in pMA Vector

    		 nucleotid sequence of ORF-1:
    GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAA AAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGA ACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGA AAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTG GGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGAT CGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATC TGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAG ACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTC AAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCA ACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGG GCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGC AGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGA TCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    GWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFM KVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQ TRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLI GGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+ShortLinker+FokI_pMA.txt



    •  His+FluA+middellinker+FokA_pMA


    His+FluA+middellinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT CTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCC GATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATA ACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAAC CAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCC GTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAG GCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAAT GGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAA AGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGG AGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF MKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKEN QTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELL IGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+middellinker+FokA_pMA.txt



    •  His+FluA+middellinker+Foki_pMA


    His+FluA+middellinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT CTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCC GATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATA ATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAAT CAGACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCC GTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAG GCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAAT GGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAA AGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCG AGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF MKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEEN QTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELL IGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+middellinker+Foki_pMA.txt



    •  His+FluA+shortlinker+FokA_pMA


    His+FluA+shortlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAG CTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGA GATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGG AGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGC CGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGG CGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTG GTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAAC AAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTAC AGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAG CACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTG TCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCT GACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTA CCGGTTAA

    AA sequence of ORF-1:

    HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVY GYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRN KHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGE MIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+shortlinker+FokA_pMA.txt





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