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Team:HKU-HKBU/protocols
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=Protocols= | =Protocols= | ||
| + | |||
| + | ==Membrane Biotinylation== | ||
| + | # Activate the membrane with methanol for 10-30seconds | ||
| + | # Balance the membrane in PBS for 5 minutes | ||
| + | # Place the membrane on a piece of filter paper. | ||
| + | # Drop the protein-biotin complex onto the membrane. | ||
| + | # Air-dry for 5 minutes. | ||
| + | # Soak in methanol for 1minutes. | ||
| + | # Place the membrane on a piece of filter paper. | ||
| + | # Air dry for 15 minutes. | ||
==Pre-culture== | ==Pre-culture== | ||
Revision as of 13:20, 12 October 2009
Contents |
Protocols
Membrane Biotinylation
- Activate the membrane with methanol for 10-30seconds
- Balance the membrane in PBS for 5 minutes
- Place the membrane on a piece of filter paper.
- Drop the protein-biotin complex onto the membrane.
- Air-dry for 5 minutes.
- Soak in methanol for 1minutes.
- Place the membrane on a piece of filter paper.
- Air dry for 15 minutes.
Pre-culture
Transformation
Bacteria Lysis
BCA Quantification Analysis
Western Blotting
Sponsors
