Team:UC Davis/Adding secretion

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Revision as of 03:23, 13 October 2009

Adding Secretion

Adding Secretion:

General model for secretion Secretion Models Why test different genes

General model for secretion system:

Advantages of having several models:

          The purpose of the secretion system is to introduce a method of excreting target proteins we wish to synthesize in the marvelous host: E.coli HB101. Through this approach of being able to secrete specific target proteins, we hope to serve as a chassis to the rest of the biological world.
        In our secretion system, we are using two genes with different sizes as our target secretion genes, GFP being short in length and Luciferase being comparably long would test our secretion system and its ability to secrete small and large proteins.
        Testing multiple different combinations; Signal Sequence alone, Signal Sequence plus ompA/INPNC and ompA/INPNC alone, would help us find the best combination for our secretion system based on their secretion ability and rank them from strongest to weakest in strength (in this specific system).

Secretion Models:
Click on a specific model for more information:

Why test different genes in our secretion system?

There is wide range of genes present in our environment. Therefore, it is important to measure our secretion system’s ability to secrete genes of various sizes.

Molecular data on some proteins

Name	Molecular Weight	Number of residues	Number of polypeptide
Cytochrome c (human )	13,000	104	1
Ribonuclease A(bovine pancreas)	13,700	124	1
Serum albumin (human)	68,500	609	1

(Page 128, third edition “Lehninger Principles of Biochemistry” by David L. Nelson and Michael M. Cox)

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-of the secretion system is to introduce a method
+<big>The purpose of the secretion system is to introduce a method
 of excreting target proteins we wish to synthesize in the marvelous
 host: E.coli
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-&nbsp;&nbsp; In our secretion system, we are using two
+&nbsp;&nbsp;&nbsp; In our secretion system, we are using two
 genes with different sizes as our target secretion genes, GFP being
 short in