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Team:Todai-Tokyo/Notebook/bread
From 2009.igem.org
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'''Methods:'''<BR> | '''Methods:'''<BR> | ||
#Clone the glucoamylase gene from Saccharomycopsis fibuligera<BR> | #Clone the glucoamylase gene from Saccharomycopsis fibuligera<BR> | ||
| - | #Clone mtlD (mannitol synthase) from E.coli and insert it in the yeast chromosome by homologous recombination | + | #Clone mtlD (mannitol synthase) from E.coli and insert it in the yeast chromosome by homologous recombination |
| - | Replace gpd gene by mtlD<BR> | + | #Replace gpd gene by mtlD<BR> |
=='''September'''== | =='''September'''== | ||
Revision as of 08:35, 13 October 2009
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Plan
Aim:Create yeast that makes sweet and low energy sugar from starch
Methods:
- Clone the glucoamylase gene from Saccharomycopsis fibuligera
- Clone mtlD (mannitol synthase) from E.coli and insert it in the yeast chromosome by homologous recombination
- Replace gpd gene by mtlD
September
- PCR of mtlD
- TA cloning of mtlD
- PCR of gpd1 promoter
October
- PCR of Glu1
- cut mtlD by XbaI and PstI
- PCR of gpd1 promoter with ExTaq
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