Team:Todai-Tokyo/Notebook/bread

From 2009.igem.org

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{{:Team:Todai-Tokyo/Template}}
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=='''Plan'''==
=='''Plan'''==
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'''Aim:'''Create yeast that makes sweet and low energy sugar from starch
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'''Aim:'''Create yeast that can be used to make sweet and low energy bread<BR>
'''Methods:'''<BR>
'''Methods:'''<BR>
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*PCR of mtlD<BR>
*PCR of mtlD<BR>
*TA cloning of mtlD<BR>
*TA cloning of mtlD<BR>
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*PCR of gpd1 promoter<BR>
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*PCR of gpd1 promoter with Pfu Ultra<BR>
=='''October'''==
=='''October'''==
*PCR of Glu1<BR>
*PCR of Glu1<BR>
 +
*TA cloning of Glu1<BR>
*cut mtlD by XbaI and PstI<BR>
*cut mtlD by XbaI and PstI<BR>
*PCR of gpd1 promoter with ExTaq<BR>
*PCR of gpd1 promoter with ExTaq<BR>
 +

Revision as of 08:41, 13 October 2009

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Plan

Aim:Create yeast that can be used to make sweet and low energy bread

Methods:

  1. Clone the glucoamylase gene from Saccharomycopsis fibuligera
  2. Clone mtlD (mannitol synthase) from E.coli and insert it in the yeast chromosome by homologous recombination
  3. Replace gpd gene by mtlD

September

  • PCR of mtlD
  • TA cloning of mtlD
  • PCR of gpd1 promoter with Pfu Ultra

October

  • PCR of Glu1
  • TA cloning of Glu1
  • cut mtlD by XbaI and PstI
  • PCR of gpd1 promoter with ExTaq






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