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Team:Todai-Tokyo/Notebook/bread
From 2009.igem.org
(Difference between revisions)
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#Replace gpd gene by mtlD<BR> | #Replace gpd gene by mtlD<BR> | ||
| - | ==''' | + | =='''~9/20'''== |
*PCR of mtlD<BR> | *PCR of mtlD<BR> | ||
| + | *PCR of gpd1 promoter<BR> | ||
*TA cloning of mtlD<BR> | *TA cloning of mtlD<BR> | ||
| + | |||
| + | =="'9/21'''== | ||
| + | *Miniprep of mtlD<BR> | ||
| + | |||
| + | =='''9/22'''== | ||
| + | *read the sequence of mtlD<BR> | ||
| + | successful | ||
| + | |||
| + | =='''9/23'''== | ||
*PCR of gpd1 promoter with Pfu Ultra<BR> | *PCR of gpd1 promoter with Pfu Ultra<BR> | ||
Revision as of 03:45, 14 October 2009
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook | Protocols | Ethics |
|---|
Contents |
Plan
Aim:Create yeast that can be used to make sweet and low energy bread
Methods:
- Clone the glucoamylase gene from Saccharomycopsis fibuligera
- Clone mtlD (mannitol synthase) from E.coli and insert it in the yeast chromosome by homologous recombination
- Replace gpd gene by mtlD
~9/20
- PCR of mtlD
- PCR of gpd1 promoter
- TA cloning of mtlD
"'9/21
- Miniprep of mtlD
9/22
- read the sequence of mtlD
successful
9/23
- PCR of gpd1 promoter with Pfu Ultra
October
- PCR of Glu1
- TA cloning of Glu1
- cut mtlD by XbaI and PstI
- PCR of gpd1 promoter with ExTaq
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