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Team:Todai-Tokyo/Notebook/bread
From 2009.igem.org
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*PCR of gpd1 promoter with Pfu Ultra<BR> | *PCR of gpd1 promoter with Pfu Ultra<BR> | ||
| + | *mtlD primers with HAtag come<BR> | ||
=='''October'''== | =='''October'''== | ||
Revision as of 03:50, 14 October 2009
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Contents |
Plan
Aim:Create yeast that can be used to make sweet and low energy bread
Methods:
- Clone the glucoamylase gene from Saccharomycopsis fibuligera
- Clone mtlD (mannitol synthase) from E.coli and insert it in the yeast chromosome by homologous recombination
- Replace gpd gene by mtlD
~9/20
- PCR of mtlD
- PCR of gpd1 promoter
- TA cloning of mtlD
"'9/21
- Miniprep of mtlD
9/22
- read the sequence of mtlD→successful
9/23
- PCR of gpd1 promoter with Pfu Ultra
- mtlD primers with HAtag come
October
- PCR of Glu1
- TA cloning of Glu1
- cut mtlD by XbaI and PstI
- PCR of gpd1 promoter with ExTaq
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