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Team:Todai-Tokyo/Notebook/bread
From 2009.igem.org
(Difference between revisions)
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*PCR of Glu1<BR> | *PCR of Glu1<BR> | ||
*TA cloning of Glu1<BR> | *TA cloning of Glu1<BR> | ||
| - | |||
*PCR of gpd1 promoter with ExTaq<BR> | *PCR of gpd1 promoter with ExTaq<BR> | ||
| + | |||
| + | =="'10/14"'== | ||
| + | *cut mtlD and plate1 7D both by EcoRI and PstI<BR> | ||
| + | *colony PCR of Glu1<BR> | ||
Revision as of 03:45, 15 October 2009
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Contents |
Plan
Aim:Create yeast that can be used to make sweet and low energy bread
Methods:
- Clone the glucoamylase gene from Saccharomycopsis fibuligera
- Clone mtlD (mannitol synthase) from E.coli and insert it in the yeast chromosome by homologous recombination
- Replace gpd gene by mtlD
~9/20
- PCR of mtlD
- PCR of gpd1 promoter
- TA cloning of mtlD
"'9/21
- Miniprep of mtlD
9/22
- read the sequence of mtlD→successful
- mtlD primers with HAtag come
9/25
- PCR of mtlD with new primer→failed
9/26
- PCR of mtlD with new primer→successful
9/27
- PCR of gpd1 promoter with Pfu Ultra
October
- PCR of Glu1
- TA cloning of Glu1
- PCR of gpd1 promoter with ExTaq
"'10/14"'
- cut mtlD and plate1 7D both by EcoRI and PstI
- colony PCR of Glu1
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