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Week Four
From 2009.igem.org
(New page: ===June 8th- 17th=== '''What are we trying to do?''' We want the bacteria to lyse, to kill itself from the inside. Well then, to do that we had to get a lysis gene in it. The thing is, ...) |
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How we tried to do this: | How we tried to do this: | ||
| - | '''[[Step 1 - Taking dry DNA from wells]]''' | + | '''[[Team:ArtScienceBangalore/Notebook/Step 1 - Taking dry DNA from wells|Step 1 - Taking dry DNA from wells]]''' |
| - | '''[[Step 2 - Transforming competent cells]]''' | + | '''[[Team:ArtScienceBangalore/Notebook/Step 2 - Transforming competent cells|Step 2 - Transforming competent cells]]''' |
'''Step 3 - Picking a single colony.''' | '''Step 3 - Picking a single colony.''' | ||
| Line 24: | Line 24: | ||
'''Step 5 - Using the resulting culture for [[mini-prep.]]''' - ''a process used to purify plasmids and yields clean, usable DNA.'' | '''Step 5 - Using the resulting culture for [[mini-prep.]]''' - ''a process used to purify plasmids and yields clean, usable DNA.'' | ||
| - | '''Step 6 - [[Digesting the DNA]]''' | + | '''Step 6 - [[Team:ArtScienceBangalore/Notebook/Digesting the DNA|Digesting the DNA]]''' |
| - | '''Step 7 - [[Gel Electrophoresis]]''' | + | '''Step 7 - [[Team:ArtScienceBangalore/Notebook/Gel Electrophoresis|Gel Electrophoresis]]''' |
| - | '''Step 8- [[Ligation]]''' | + | '''Step 8- [[Team:ArtScienceBangalore/Notebook/Ligation|Ligation]]''' |
| - | '''Step 9- [[Transformation and Inoculation]]''' | + | '''Step 9- [[Team:ArtScienceBangalore/Notebook/Transformation and Inoculation|Transformation and Inoculation]]''' |
===June 9th=== | ===June 9th=== | ||
| - | [[The June 9th Image Gallery]] | + | [[Team:ArtScienceBangalore/Notebook/The June 9th Image Gallery|The June 9th Image Gallery]] |
Images of the various results attained: | Images of the various results attained: | ||
Revision as of 17:04, 14 October 2009
June 8th- 17th
What are we trying to do?
We want the bacteria to lyse, to kill itself from the inside.
Well then, to do that we had to get a lysis gene in it. The thing is, with the lysis gene needs to be turned on by this specific substance called a promoter
There are two kinds of promoters, ones that make the gene they're attached to , be 'on' all the time. Then, you have ones that turn on when you want them to, in our case, when you add a mystery enzyme.
Our Step-wise Process:
How we tried to do this:
Step 1 - Taking dry DNA from wells
Step 2 - Transforming competent cells
Step 3 - Picking a single colony.
Step 4 - Inoculating broth with Ampicillin-R (an antibiotic) and letting it grow for 18 hours.
Step 5 - Using the resulting culture for mini-prep. - a process used to purify plasmids and yields clean, usable DNA.
Step 6 - Digesting the DNA
Step 7 - Gel Electrophoresis
Step 8- Ligation
Step 9- Transformation and Inoculation
June 9th
Images of the various results attained:
 Agarose gel electrophoresis image of digested K112808 |
 Failure 1 |
 The pBAD Insert |
 The Failed Ligation Attempt |
