Team:Berkeley Wetlab

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<b>[[Assay Protocols | Functional Assays]] * [[Assay Results | Results]] * [[ Recipes| Recipes]] [[ NoteBooks | NoteBooks ]] </b>
<b>[[Assay Protocols | Functional Assays]] * [[Assay Results | Results]] * [[ Recipes| Recipes]] [[ NoteBooks | NoteBooks ]] </b>
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Revision as of 06:36, 15 October 2009

Functional Assays * Results * Recipes NoteBooks

Preliminary project description


The University of California Berkeley iGEM team is proposing to expand the design space of synthetic biology by exploring novel applications of cell surface display within Escherichia coli, the gold standard organism for bacterial engineering. The team envisions a bottom-up design scheme in order to tackle this engineering problem in a well organized, modular fashion. In order to overcome the challenges of engineering Escherichia coli cell surface display, a high throughput, automated, combinatorial strategy is employed to control the system. 



















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The University of California Berkeley iGem team is proposing to expand the design space of synthetic biology by exploring novel applications of cell surface display within Escherichia coli, the gold standard organism for bacterial engineering. The team envisions a bottom-up design scheme in order to tackle this engineering problem in a well organized, modular fashion. In order to overcome the challenges of engineering Escherichia coli cell surface display, a high throughput, automated, combinatorial strategy is employed to control the system.

Support for Berkeley iGEM 2009 was generously provided by SynBERC and The Camille and Henry Dreyfus Foundation, Inc.

 

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The System's Components
<a href="http://parts.mit.edu/igem07/index.php/BerkiGEM2007Present1"><img src="Berkeley_UC_files/Berk-Icon-Oxygen.png" width="121" border="0" height="121"></a> <a href="http://parts.mit.edu/igem07/index.php/BerkiGEM2007Present1">Oxygen Transport</a>

Our system is

designed to produce Hemoglobin, Heme, and the necessary chaperones and detoxifying agents to promote the transport of oxygen throughout the bloodstream.  We also investigated alternates to hemoglobin and other strategies

for its production.
<a href="http://parts.mit.edu/igem07/index.php/BerkiGEM2007Present4"><img src="Berkeley_UC_files/Berk-Icon-Chassis.png" width="121" border="0" height="121"></a> <a href="http://parts.mit.edu/igem07/index.php/BerkiGEM2007Present4">The Chassis</a>

Our bacterial

chassis has been heavily modified to remove its sepsis-inducing toxicity, immunogenic factors, and ability to grow within the bloodstream, as well as promote its ability to last longer in the bloodstream by masking it from the immune

system.
<a href="http://parts.mit.edu/igem07/index.php/BerkiGEM2007Present3"><img src="Berkeley_UC_files/Berk-Icon-Controller.png" width="121" border="0" height="121"></a> <a href="http://parts.mit.edu/igem07/index.php/BerkiGEM2007Present3">The Controller</a>

The Controller

is an integrated genetic circuit comprised of two plasmids that allows stable maintenance of the system's various operons on a large single-copy plasmid in a dormant state. Upon induction, the copy number of the operons and their transcription increase 100-fold resulting in a

dramatic increase in protein expression.
<a href="http://parts.mit.edu/igem07/index.php/BerkiGEM2007Present5"><img src="Berkeley_UC_files/Berk-Icon-Self-Destruct.png" width="121" border="0" height="121"></a> <a href="http://parts.mit.edu/igem07/index.php/BerkiGEM2007Present5">Genetic Self-Destruct</a>

To

prevent chance of infection or unwanted proliferation after hemoglobin production, we have engineered a genetic self-destruct mechanism whereby when induced, the bacterial cell will express a genetic material-degrading toxin which

kills the cell, but leaves it physically intact.
<a href="http://parts.mit.edu/igem07/index.php/BerkiGEM2007Present2"><img src="Berkeley_UC_files/Berk-Icon-Freeze-Drying.png" width="121" border="0" height="121"></a> <a href="http://parts.mit.edu/igem07/index.php/BerkiGEM2007Present2">Freeze Drying</a>

To

enable preservation of our bacteria for prolonged periods, we are including the ability to produce the compounds hydroxyectoine and trehalose that will enable our bacteria to survive freeze-drying intact.  This will dramatically

increase shelf-life and decrease transport costs.


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<a href="http://parts.mit.edu/igem07/index.php/BerkiGEM2007Present6"><img src="Berkeley_UC_files/Berk-Icon-Small-Patents.png" width="83" border="0" height="83"></a> <a href="http://parts.mit.edu/igem07/index.php/BerkiGEM2007Present6">Human Practices</a>

An

examination of the landscape of patents and the patentablilty of Bactoblood where its parts are in an open source forum and the challenges associated with current patenting practices for synthetic

biology.
<a href="http://parts.mit.edu/igem07/index.php/Berkeley_Individual_Contributions"><img src="Berkeley_UC_files/Berk-Icon-Small-References.png" width="83" border="0" height="83"></a> <a href="http://parts.mit.edu/igem07/index.php/Berkeley_Individual_Contributions">Individual Contributions</a>

The specific contributions made by each team member and advisor.
<a href="http://parts.mit.edu/igem07/index.php/BerkiGEM2007_Resources"><img src="Berkeley_UC_files/Berk-Icon-Small-Resources.png" width="83" border="0" height="83"></a> <a href="http://parts.mit.edu/igem07/index.php/BerkiGEM2007_Resources">Team Resources</a>

Organizational spreadsheets, useful tools and links.
<a href="http://parts.mit.edu/igem07/index.php/BerkiGEM2007_Notebooks"><img src="Berkeley_UC_files/Berk-Icon-Small-Notebooks.png" width="83" border="0" height="83"></a> <a href="http://parts.mit.edu/igem07/index.php/BerkiGEM2007_Notebooks">Team Notebooks</a>

The team members' daily logs of our research.


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Team Members
Advisors

<a href="http://parts.mit.edu/igem07/index.php/John_Dueber" title="John Dueber"> John Dueber</a> • <a href="http://www.openwetware.org/wiki/User:JCAnderson" title="http://www.openwetware.org/wiki/User:JCAnderson" rel="nofollow"> Christopher Anderson</a> • <a href="http://genomics.lbl.gov/" title="http://genomics.lbl.gov/" rel="nofollow"> Adam Arkin</a> • <a href="https://keaslinglab.lbl.gov/wiki/index.php/Main_Page" title="https://keaslinglab.lbl.gov/wiki/index.php/Main Page" rel="nofollow"> Jay Keasling</a>

Teaching Assistants
<a href="http://parts.mit.edu/igem07/index.php/Farnaz_Nowroozi" title="Farnaz Nowroozi"> Farnaz Nowroozi</a> • <a href="http://parts.mit.edu/igem07/index.php/Amin_Hajimorad" title="Amin Hajimorad"> Amin Hajimorad</a> • <a href="http://parts.mit.edu/igem07/index.php/Rickey_Bonds" title="Rickey Bonds"> Rickey Bonds</a>

Support
<a href="http://parts.mit.edu/igem07/index.php/Kate%20Spohr" title="Kate Spohr"> Kate Spohr</a> • <a href="http://parts.mit.edu/igem07/index.php/Kevin%20Costa" title="Kevin Costa"> Kevin Costa</a> • <a href="http://parts.mit.edu/igem07/index.php/Gwyneth%20Terry" title="Gwyneth Terry"> Gwyneth Terry</a>

Undergraduate Researchers
<a href="http://parts.mit.edu/igem07/index.php/Arthur_Yu" title="Arthur Yu"> Arthur Yu</a> • <a href="http://parts.mit.edu/igem07/index.php/Austin_Day" title="Austin Day"> Austin Day</a> • <a href="http://parts.mit.edu/igem07/index.php/David_Tulga" title="David Tulga"> David Tulga</a> • <a href="http://parts.mit.edu/igem07/index.php/Kristin_Doan" title="Kristin Doan"> Kristin Doan</a> • <a href="http://parts.mit.edu/igem07/index.php/Samantha_Liang" title="Samantha Liang"> Samantha Liang</a> • <a href="http://parts.mit.edu/igem07/index.php/Vaibhavi_Umesh" title="Vaibhavi Umesh"> Vaibhavi Umesh</a> • <a href="http://parts.mit.edu/igem07/index.php/Kristin_Fuller" title="Kristin Fuller"> Kristin Fuller</a>

High School Students
<a href="http://parts.mit.edu/igem07/index.php/Vincent_Parker" title="Vincent Parker"> Vincent Parker</a> • <a href="http://parts.mit.edu/igem07/index.php/Nhu_Nguyen" title="Nhu Nguyen"> Nhu Nguyen</a> • <a href="http://parts.mit.edu/igem07/index.php/Hannah_Cole" title="Hannah Cole"> Hannah Cole</a>


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