WTM
From 2009.igem.org
(→Wild Type Molecular Model) |
(→Wild Type Molecular Model) |
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</center> | </center> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | Chemical Reactions | ||
+ | |||
+ | </head> | ||
+ | <body link="#0000d4" vlink="#993366"> | ||
+ | <table border=1 cellpadding=0 cellspacing=0 width=961 style='border-collapse: | ||
+ | collapse;table-layout:fixed'> | ||
+ | <col width=209> | ||
+ | <col width=752> | ||
+ | |||
+ | <tr height=13> | ||
+ | <td height=13 width=209>Name</td> | ||
+ | <td width=752>Reaction</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>PoliI transcription</td> | ||
+ | <td>DNAclassI -> DNAclassI + PolycistronicmRNAI</td> | ||
+ | |||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>PoliIII transcription</td> | ||
+ | <td>DNAClass2b + T7RNApol -> DNAClass2b + PolycistronicmRNAIII + T7RNApol</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>PoliIV transcription</td> | ||
+ | |||
+ | <td>DNAClass3a + T7RNApol -> DNAClass3a + PolycistronicmRNAIV + T7RNApol</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>PoliV transcription</td> | ||
+ | <td>DNAClass3a + T7RNApol -> DNAClass3a + PolycistronicmRNAV + T7RNApol</td> | ||
+ | |||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>PoliVI transcription</td> | ||
+ | <td>DNAClass3a + T7RNApol -> DNAClass3a + PolycistronicmRNAVI + T7RNApol</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>PoliVII transcription</td> | ||
+ | |||
+ | <td>DNAClass3b + T7RNApol -> DNAClass3b + PolycistronicmRNAVII + T7RNApol</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>PoliVIII transcription</td> | ||
+ | <td>DNAClass3c + T7RNApol -> DNAClass3c + PolycistronicmRNAVIII + T7RNApol</td> | ||
+ | |||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>PoliIX transcription</td> | ||
+ | <td>DNAClass3c + T7RNApol -> DNAClass3c + T7RNApol + PolycistronicmRNAIX</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>T7RNApol translation</td> | ||
+ | |||
+ | <td>PolycistronicmRNAI -> PolycistronicmRNAI + T7RNApol</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>T7DNApol translation</td> | ||
+ | <td>PolycistronicmRNAIII -> PolycistronicmRNAIII + T7DNApol</td> | ||
+ | |||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>HeadTail translation</td> | ||
+ | <td>PolycistronicmRNAIV -> PolycistronicmRNAIV + HeadTail</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>CapsidAssembly_IV translation</td> | ||
+ | |||
+ | <td>PolycistronicmRNAIV -> PolycistronicmRNAIV + CapsidAssembly</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>ABproteins_IV translation</td> | ||
+ | <td>PolycistronicmRNAIV -> PolycistronicmRNAIV + ABProteins</td> | ||
+ | |||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>CapsidAssembly_V translation</td> | ||
+ | <td>PolycistronicmRNAV -> PolycistronicmRNAV + CapsidAssembly</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>ABproteins_V translation</td> | ||
+ | |||
+ | <td>PolycistronicmRNAV -> PolycistronicmRNAV + ABProteins</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>ABproteins_VI translation</td> | ||
+ | <td>PolycistronicmRNAVI -> PolycistronicmRNAVI + ABProteins</td> | ||
+ | |||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>ABproteins_VII translation</td> | ||
+ | <td>PolycistronicmRNAVII -> PolycistronicmRNAVII + ABProteins</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>TailA_VII translation</td> | ||
+ | |||
+ | <td>PolycistronicmRNAVII -> PolycistronicmRNAVII + TailA</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>TailB_VII translation</td> | ||
+ | <td>PolycistronicmRNAVII -> PolycistronicmRNAVII + TailB</td> | ||
+ | |||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>CoreA translation</td> | ||
+ | <td>PolycistronicmRNAVIII -> PolycistronicmRNAVIII + CoreA</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>CoreB translation</td> | ||
+ | |||
+ | <td>PolycistronicmRNAVIII -> PolycistronicmRNAVIII + CoreB</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>CoreC translation</td> | ||
+ | <td>PolycistronicmRNAVIII -> PolycistronicmRNAVIII + CoreC</td> | ||
+ | |||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>CoreD translation</td> | ||
+ | <td>PolycistronicmRNAVIII -> PolycistronicmRNAVIII + CoreD</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>TailFiber_VIII translation</td> | ||
+ | |||
+ | <td>PolycistronicmRNAVIII -> PolycistronicmRNAVIII + TailFiber</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>Procapsid formation</td> | ||
+ | <td>417 ABProteins -> Procapsid</td> | ||
+ | |||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>DNA packaging/Final Assembly</td> | ||
+ | <td>12 HeadTail + 137 CapsidAssembly + Procapsid + 18 TailA + 6 TailB + 33 | ||
+ | CoreA + 18 CoreB + 12 CoreC + 3 CoreD + 18 TailFiber + T7DNA -> T7</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>TailFiber_IX translation</td> | ||
+ | |||
+ | <td>PolycistronicmRNAIX -> PolycistronicmRNAIX + TailFiber</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>PolycistronicmRNAIX degradation</td> | ||
+ | <td>PolycistronicmRNAIX -> null</td> | ||
+ | |||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>Phage Replication</td> | ||
+ | <td>T7DNApol -> T7DNApol + T7DNA</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>PolycistronicmRNAI degradation</td> | ||
+ | |||
+ | <td>PolycistronicmRNAI -> null</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>PolycistronicmRNAIII degradation</td> | ||
+ | <td>PolycistronicmRNAIII -> null</td> | ||
+ | |||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>PolycistronicmRNAIV degradation</td> | ||
+ | <td>PolycistronicmRNAIV -> null</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>PolycistronicmRNAV degradation</td> | ||
+ | |||
+ | <td>PolycistronicmRNAV -> null</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>PolycistronicmRNAVI degradation</td> | ||
+ | <td>PolycistronicmRNAVI -> null</td> | ||
+ | |||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>PolycistronicmRNAVII degradation</td> | ||
+ | <td>PolycistronicmRNAVII -> null</td> | ||
+ | </tr> | ||
+ | <tr height=13> | ||
+ | <td height=13>PolycistronicmRNAVIII degradation</td> | ||
+ | |||
+ | <td>PolycistronicmRNAVIII -> null</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </body> | ||
</html> | </html> |
Revision as of 22:15, 15 October 2009
Wild Type Molecular Model
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Name | Reaction |
PoliI transcription | DNAclassI -> DNAclassI + PolycistronicmRNAI |
PoliIII transcription | DNAClass2b + T7RNApol -> DNAClass2b + PolycistronicmRNAIII + T7RNApol |
PoliIV transcription | DNAClass3a + T7RNApol -> DNAClass3a + PolycistronicmRNAIV + T7RNApol |
PoliV transcription | DNAClass3a + T7RNApol -> DNAClass3a + PolycistronicmRNAV + T7RNApol |
PoliVI transcription | DNAClass3a + T7RNApol -> DNAClass3a + PolycistronicmRNAVI + T7RNApol |
PoliVII transcription | DNAClass3b + T7RNApol -> DNAClass3b + PolycistronicmRNAVII + T7RNApol |
PoliVIII transcription | DNAClass3c + T7RNApol -> DNAClass3c + PolycistronicmRNAVIII + T7RNApol |
PoliIX transcription | DNAClass3c + T7RNApol -> DNAClass3c + T7RNApol + PolycistronicmRNAIX |
T7RNApol translation | PolycistronicmRNAI -> PolycistronicmRNAI + T7RNApol |
T7DNApol translation | PolycistronicmRNAIII -> PolycistronicmRNAIII + T7DNApol |
HeadTail translation | PolycistronicmRNAIV -> PolycistronicmRNAIV + HeadTail |
CapsidAssembly_IV translation | PolycistronicmRNAIV -> PolycistronicmRNAIV + CapsidAssembly |
ABproteins_IV translation | PolycistronicmRNAIV -> PolycistronicmRNAIV + ABProteins |
CapsidAssembly_V translation | PolycistronicmRNAV -> PolycistronicmRNAV + CapsidAssembly |
ABproteins_V translation | PolycistronicmRNAV -> PolycistronicmRNAV + ABProteins |
ABproteins_VI translation | PolycistronicmRNAVI -> PolycistronicmRNAVI + ABProteins |
ABproteins_VII translation | PolycistronicmRNAVII -> PolycistronicmRNAVII + ABProteins |
TailA_VII translation | PolycistronicmRNAVII -> PolycistronicmRNAVII + TailA |
TailB_VII translation | PolycistronicmRNAVII -> PolycistronicmRNAVII + TailB |
CoreA translation | PolycistronicmRNAVIII -> PolycistronicmRNAVIII + CoreA |
CoreB translation | PolycistronicmRNAVIII -> PolycistronicmRNAVIII + CoreB |
CoreC translation | PolycistronicmRNAVIII -> PolycistronicmRNAVIII + CoreC |
CoreD translation | PolycistronicmRNAVIII -> PolycistronicmRNAVIII + CoreD |
TailFiber_VIII translation | PolycistronicmRNAVIII -> PolycistronicmRNAVIII + TailFiber |
Procapsid formation | 417 ABProteins -> Procapsid |
DNA packaging/Final Assembly | 12 HeadTail + 137 CapsidAssembly + Procapsid + 18 TailA + 6 TailB + 33 CoreA + 18 CoreB + 12 CoreC + 3 CoreD + 18 TailFiber + T7DNA -> T7 |
TailFiber_IX translation | PolycistronicmRNAIX -> PolycistronicmRNAIX + TailFiber |
PolycistronicmRNAIX degradation | PolycistronicmRNAIX -> null |
Phage Replication | T7DNApol -> T7DNApol + T7DNA |
PolycistronicmRNAI degradation | PolycistronicmRNAI -> null |
PolycistronicmRNAIII degradation | PolycistronicmRNAIII -> null |
PolycistronicmRNAIV degradation | PolycistronicmRNAIV -> null |
PolycistronicmRNAV degradation | PolycistronicmRNAV -> null |
PolycistronicmRNAVI degradation | PolycistronicmRNAVI -> null |
PolycistronicmRNAVII degradation | PolycistronicmRNAVII -> null |
PolycistronicmRNAVIII degradation | PolycistronicmRNAVIII -> null |