Team:SDU-Denmark/Project

From 2009.igem.org

(Difference between revisions)
(Update on the planning of experiments)
Line 19: Line 19:
[[Image:SDU-Denmark-Staphylococcus_aureus%2C_50%2C000x%2C_USDA%2C_ARS%2C_EMU.jpg|200px|thumb|right| Staphyloccocus Aureus]]
[[Image:SDU-Denmark-Staphylococcus_aureus%2C_50%2C000x%2C_USDA%2C_ARS%2C_EMU.jpg|200px|thumb|right| Staphyloccocus Aureus]]
-
== Project Details==
+
=='''Initial phase planning'''==
 +
=== Preliminary Project Details===
 +
The details we need to settle on soon (eg. by Thursday) are:
 +
1) Which bacteria is the producer in the bacto bandage (suggestions: E. coli, Lactococcus, ??)
 +
2) Identifying the natural producer of the quorum quenching signal (QQS)
-
=== Part 2 ===
+
3) Which proteins should be expressed
 +
4) How should expression be regulated
 +
5) How do we retain plasmid stability in the bactobandage
 +
6) More?
-
=== The Experiments ===
+
=== Initial Experimental Considerations ===
 +
Before we can get started a number of experimental procedures need to be discussed and/or protocols need to be identified:
 +
Initial lab work:
-
=== Part 3 ===
+
1) Making competent E. Coli for our freezer
 +
2) Amplification of basic parts in E. Coli
 +
3) Plasmid purification (maxi prep og mini prep)
 +
 +
4) Growth and handling of reciever bacteria
 +
 +
5) Identify and amplify our favourite plasmid backbones
 +
 +
 +
Biobrick expression:
 +
 +
1) Planning and ordering primers
 +
 +
2) Amplification of target gene from chromosomal DNA (PCR) 
 +
 +
3) Manipulation of biobrick part and composite parts
 +
 +
4) Transformation/electroporation into producer bacteria
 +
 +
 +
Testing output:
 +
 +
1) Western blot (protein)
 +
 +
2) Northern blot (RNA)
 +
 +
3) Promoter LaxZ or XFP fusions
 +
 +
4) RT-PCR
 +
 +
5) Sequencing (a service we buy)
 +
 +
6) More???
 +
 +
 +
== Experiments ==
== Results ==
== Results ==

Revision as of 10:38, 21 June 2009

Home The Team The Project Parts Submitted to the Registry Modeling Notebook


Contents

Overall project

Bactobandage

Inhibition of quorum-sensing mediated biofilm-formation in staphylococcus aureus.

We're going to stop Staphylococcus aureus infections (not colonization) by inhibiting the quorum sensing-dependent biofilm formation

Staphyloccocus Aureus

Initial phase planning

Preliminary Project Details

The details we need to settle on soon (eg. by Thursday) are:

1) Which bacteria is the producer in the bacto bandage (suggestions: E. coli, Lactococcus, ??)

2) Identifying the natural producer of the quorum quenching signal (QQS)

3) Which proteins should be expressed

4) How should expression be regulated

5) How do we retain plasmid stability in the bactobandage

6) More?


Initial Experimental Considerations

Before we can get started a number of experimental procedures need to be discussed and/or protocols need to be identified:


Initial lab work:

1) Making competent E. Coli for our freezer

2) Amplification of basic parts in E. Coli

3) Plasmid purification (maxi prep og mini prep)

4) Growth and handling of reciever bacteria

5) Identify and amplify our favourite plasmid backbones


Biobrick expression:

1) Planning and ordering primers

2) Amplification of target gene from chromosomal DNA (PCR)

3) Manipulation of biobrick part and composite parts

4) Transformation/electroporation into producer bacteria


Testing output:

1) Western blot (protein)

2) Northern blot (RNA)

3) Promoter LaxZ or XFP fusions

4) RT-PCR

5) Sequencing (a service we buy)

6) More???


Experiments

Results

Retrieved from "http://2009.igem.org/Team:SDU-Denmark/Project"