HKU-HKBU/29 July 2009
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(New page: =====Co-transformation Transform two plasmids (A+, C+) to MG3. ① Plac-CheZ-His-TT C+ ② Lac Iq A+===== =====14:10 Subculture to warm room (37℃), shaking for 1.5h.===== =====15:40...)
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(New page: =====Co-transformation Transform two plasmids (A+, C+) to MG3. ① Plac-CheZ-His-TT C+ ② Lac Iq A+===== =====14:10 Subculture to warm room (37℃), shaking for 1.5h.===== =====15:40...)
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Revision as of 14:27, 16 October 2009
=====Co-transformation Transform two plasmids (A+, C+) to MG3. ① Plac-CheZ-His-TT C+ ② Lac Iq A+=====
14:10 Subculture to warm room (37℃), shaking for 1.5h.
=====15:40 take the subculture bacteria out from the warm room. Freeze the bacteria in 0℃ ice water for 15min. Centrifuge in 4000rmp at 0℃ for 2min. Decent supernatant=====