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| Line 148: |
Line 148: |
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| | | | |
| - | <a name="2"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> His+FluA+Split+Foki_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| | | | |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/3/37/Freiburg09_His%2BFluA%2BSplit%2BFokI_pMA.jpg" name="His+FluA+SplitLinker+Foki in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">His+FluA+SplitLinker+Foki in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG
| |
| - | CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
| |
| - | ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
| |
| - | GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
| |
| - | GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG
| |
| - | CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC
| |
| - | GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA
| |
| - | CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG
| |
| - | GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
| |
| - | GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
| |
| - | CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA
| |
| - | ACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA
| |
| - | GTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCT
| |
| - | GCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGA
| |
| - | TCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAG
| |
| - | TTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCG
| |
| - | TAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCG
| |
| - | TTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGT
| |
| - | CAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAA
| |
| - | ACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCG
| |
| - | AGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCC
| |
| - | CAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAG
| |
| - | TGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGA
| |
| - | CTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACC
| |
| - | GGTTAA
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP<br>
| |
| - | EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY<br>
| |
| - | IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA<br>
| |
| - | CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ<br>
| |
| - | DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG<br>
| |
| - | QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN<br>
| |
| - | SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG<br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/d/d8/Freiburg09_His%2BFluA%2BSplit%2BFokI_pMA.txt">His+FluA+Split+Foki_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - | </div><a name="3"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> His+Dig+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
| |
| - |
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/3/34/Freiburg09_His%2BDig%2BSplit%2BFokA_pMA.JPG" name="His+Dig+Split+FokA in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">His+Dig+Split+FokA in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG
| |
| - | CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
| |
| - | ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
| |
| - | GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
| |
| - | GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG
| |
| - | CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC
| |
| - | ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA
| |
| - | CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG
| |
| - | GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
| |
| - | GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
| |
| - | CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA
| |
| - | ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACCAGCC
| |
| - | TGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAA
| |
| - | ATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATG
| |
| - | TGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAA
| |
| - | GACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGG
| |
| - | CTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTT
| |
| - | ATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCC
| |
| - | TATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCG
| |
| - | TTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAAT
| |
| - | GGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTG
| |
| - | AGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCA
| |
| - | TAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTG
| |
| - | GCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGT
| |
| - | AAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
| |
| - | EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
| |
| - | IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
| |
| - | CKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE
| |
| - | LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKA
| |
| - | YSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA
| |
| - | QLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/d/dd/Freiburg09_His%2BDig%2BSplit%2BFokA_pMA.txt">His+Dig+Split+FokA_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - | </div><a name="4"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> DsbA+His+Dig+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/4/4f/Freiburg09_DsbA%2BHis%2BDig%2BSplit%2BFokA_pMA.JPG" name="DsbA+His+Dig+Split+FokA in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">DsbA+His+Dig+Split+FokA in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC
| |
| - | ATCGGCGACCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACG
| |
| - | ACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAG
| |
| - | TACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAA
| |
| - | GTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGA
| |
| - | AGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGC
| |
| - | ACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTA
| |
| - | CACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCG
| |
| - | ACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAG
| |
| - | AAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGAC
| |
| - | CGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGG
| |
| - | TGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAA
| |
| - | GTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACC
| |
| - | AGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCG
| |
| - | GCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAA
| |
| - | TATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTAC
| |
| - | CCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCT
| |
| - | ATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCC
| |
| - | ATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAA
| |
| - | AGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGC
| |
| - | AGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAAC
| |
| - | GAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTT
| |
| - | CGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGA
| |
| - | ACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTG
| |
| - | ATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCG
| |
| - | CCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGMKKIWLALAGLVLAFSASATGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAA
| |
| - | YPDHITKYGKCGWAEYTPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIG
| |
| - | GVTQVGVSNVLSTDNKNYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIG
| |
| - | SPVVDSQKLVYSDFSEAACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEE
| |
| - | KKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGA
| |
| - | IYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSV
| |
| - | TEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKF
| |
| - | NNGEINFTG
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/4/4d/Freiburg09_DsbA%2BHis%2BDig%2BSplit%2BFokA_pMA.txt">DsbA+His+Dig+Split+FokA_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - | </div><a name="5"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> Strep+FluA+ShortLinker+FokI_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/0/03/Freiburg09_Strep%2BFluA%2BShortLinker%2BFokI_pMA.jpg" name="Strep+FluA+ShortLinker+FokI in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">Strep+FluA+ShortLinker+FokI in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCC
| |
| - | GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
| |
| - | CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
| |
| - | ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
| |
| - | GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
| |
| - | GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG
| |
| - | CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC
| |
| - | GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA
| |
| - | CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG
| |
| - | GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
| |
| - | GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
| |
| - | CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA
| |
| - | ACAACACCGGCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAA
| |
| - | AAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGA
| |
| - | ACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGA
| |
| - | AAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTG
| |
| - | GGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGAT
| |
| - | CGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATC
| |
| - | TGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAG
| |
| - | ACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTC
| |
| - | AAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCA
| |
| - | ACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGG
| |
| - | GCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGC
| |
| - | AGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGA
| |
| - | TCAATTTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
| |
| - | EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
| |
| - | IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
| |
| - | CKVNNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFM
| |
| - | KVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQ
| |
| - | TRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLI
| |
| - | GGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/7/7e/Freiburg09_Strep%2BFluA%2BShortLinker%2BFokI_pMA.txt">Strep+FluA+ShortLinker+FokI_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - | </div><a name="6"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> His+FluA+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/a/ad/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bmiddellinker%2BFokA_pMA.jpg" name="His+FluA+middellinker+FokA in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">His+FluA+middellinker+FokA in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGC
| |
| - | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| |
| - | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| |
| - | GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
| |
| - | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
| |
| - | CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
| |
| - | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
| |
| - | TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
| |
| - | CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
| |
| - | ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| |
| - | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| |
| - | ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
| |
| - | GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG
| |
| - | AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT
| |
| - | CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA
| |
| - | TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT
| |
| - | CTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCC
| |
| - | GATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATA
| |
| - | ACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAAC
| |
| - | CAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCC
| |
| - | GTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAG
| |
| - | GCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAAT
| |
| - | GGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAA
| |
| - | AGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGG
| |
| - | AGATCAATTTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
| |
| - | SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
| |
| - | YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| |
| - | NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF
| |
| - | MKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKEN
| |
| - | QTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELL
| |
| - | IGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/7/78/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFokA_pMA.txt">His+FluA+middellinker+FokA_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - | </div><a name="7"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> His+FluA+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/c/ce/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.jpg" name="His+FluA+middellinker+Foki in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">His+FluA+middellinker+Foki in pMA Vector</p><br>
| |
| - | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243014">link to part registry</a>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGC
| |
| - | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| |
| - | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| |
| - | GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
| |
| - | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
| |
| - | CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
| |
| - | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
| |
| - | TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
| |
| - | CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
| |
| - | ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| |
| - | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| |
| - | ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
| |
| - | GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG
| |
| - | AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT
| |
| - | CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA
| |
| - | TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT
| |
| - | CTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCC
| |
| - | GATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATA
| |
| - | ATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAAT
| |
| - | CAGACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCC
| |
| - | GTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAG
| |
| - | GCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAAT
| |
| - | GGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAA
| |
| - | AGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCG
| |
| - | AGATCAATTTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
| |
| - | SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
| |
| - | YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| |
| - | NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF
| |
| - | MKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEEN
| |
| - | QTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELL
| |
| - | IGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/9/92/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.txt">His+FluA+middellinker+Foki_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - | </div><a name="8"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> His+FluA+shortlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/d/d4/Freiburg09_FluA%2BHis%2Bshortlinker%2BFokA_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bshortlinker%2BFokA_pMA.jpg" name="His+FluA+shortlinker+FokA in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">His+FluA+shortlinker+FokA in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGC
| |
| - | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| |
| - | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| |
| - | GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
| |
| - | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
| |
| - | CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
| |
| - | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
| |
| - | TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
| |
| - | CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
| |
| - | ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| |
| - | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| |
| - | ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
| |
| - | GCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAG
| |
| - | CTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGA
| |
| - | GATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGG
| |
| - | AGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGC
| |
| - | CGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGG
| |
| - | CGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTG
| |
| - | GTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAAC
| |
| - | AAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTAC
| |
| - | AGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAG
| |
| - | CACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTG
| |
| - | TCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCT
| |
| - | GACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTA
| |
| - | CCGGTTAA
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
| |
| - | SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
| |
| - | YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| |
| - | NNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVY
| |
| - | GYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRN
| |
| - | KHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGE
| |
| - | MIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/4/46/Freiburg09_FluA%2BHis%2Bshortlinker%2BFokA_pMA.txt">His+FluA+shortlinker+FokA_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - | </div><a name="9"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> His+FluA+shortlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/b/bc/Freiburg09_FluA%2BHis%2Bshortlinker%2BFokI_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bshortlinker%2BFokI_pMA.jpg" name="His+FluA+shortlinker+Foki in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">His+FluA+shortlinker+Foki in pMA Vector</p><br>
| |
| - | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243013">link to part registry</a>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGC
| |
| - | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| |
| - | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| |
| - | GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
| |
| - | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
| |
| - | CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
| |
| - | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
| |
| - | TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
| |
| - | CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
| |
| - | ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| |
| - | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| |
| - | ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
| |
| - | GCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAG
| |
| - | CTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGA
| |
| - | GATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGG
| |
| - | AGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGC
| |
| - | CGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGG
| |
| - | CGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTG
| |
| - | GTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAAC
| |
| - | AAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCAC
| |
| - | CGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAG
| |
| - | CCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTG
| |
| - | AGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCT
| |
| - | GACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTA
| |
| - | CCGGTTAA
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
| |
| - | SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
| |
| - | YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| |
| - | NNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVY
| |
| - | GYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQTRN
| |
| - | KHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLIGGE
| |
| - | MIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/7/7d/Freiburg09_FluA%2BHis%2Bshortlinker%2BFokI_pMA.txt">His+FluA+shortlinker+Foki_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - | </div><a name="10"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> His+Dig+longlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/7/7d/Freiburg09_His%2BDig%2Blonglinker%2BFokA_pMA.jpg/600px-Freiburg09_His%2BDig%2Blonglinker%2BFokA_pMA.jpg" name="His+Dig+longlinker+FokA in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">His+Dig+longlinker+FokA in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGC
| |
| - | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| |
| - | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| |
| - | GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
| |
| - | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
| |
| - | CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
| |
| - | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
| |
| - | GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
| |
| - | CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
| |
| - | ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| |
| - | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| |
| - | ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
| |
| - | ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT
| |
| - | TCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATC
| |
| - | CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA
| |
| - | TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG
| |
| - | ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG
| |
| - | TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT
| |
| - | ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA
| |
| - | ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG
| |
| - | TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG
| |
| - | TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT
| |
| - | AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT
| |
| - | TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA
| |
| - | CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT
| |
| - | TTTACCGGTTAA
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
| |
| - | SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
| |
| - | YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| |
| - | NNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS
| |
| - | TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP
| |
| - | IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK
| |
| - | TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/0/07/Freiburg09_His%2BDig%2Blonglinker%2BFokA_pMA.txt">His+Dig+longlinker+FokA_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - | </div><a name="11"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> His+Dig+longlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/6/64/Freiburg09_His%2BDig%2Blonglinker%2BFoki_pMA.JPG/600px-Freiburg09_His%2BDig%2Blonglinker%2BFoki_pMA.JPG" name="His+Dig+longlinker+Foki in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">His+Dig+longlinker+Foki in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGC
| |
| - | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| |
| - | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| |
| - | GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
| |
| - | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
| |
| - | CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
| |
| - | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
| |
| - | GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
| |
| - | CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
| |
| - | ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| |
| - | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| |
| - | ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
| |
| - | ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT
| |
| - | TCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATC
| |
| - | CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA
| |
| - | TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG
| |
| - | ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG
| |
| - | TAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATT
| |
| - | ATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCG
| |
| - | ATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCG
| |
| - | TAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTG
| |
| - | TCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTAT
| |
| - | AAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGT
| |
| - | GCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCA
| |
| - | CCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAAT
| |
| - | TTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
| |
| - | SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
| |
| - | YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| |
| - | NNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS
| |
| - | TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLP
| |
| - | IGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHI
| |
| - | TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/e/ef/Freiburg09_His%2BDig%2Blonglinker%2BFoki_pMA.txt">His+Dig+longlinker+Foki_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - |
| |
| - | </div><a name="12"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> Strep+FluA+Split+Foki_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/f/fa/Freiburg09_Strep%2BFluA%2BSplit%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2BSplit%2BFokI_pMA.jpg" name="Strep+FluA+Split+Foki in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">Strep+FluA+Split+Foki in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCC
| |
| - | GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
| |
| - | CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
| |
| - | ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
| |
| - | GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
| |
| - | GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG
| |
| - | CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC
| |
| - | GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA
| |
| - | CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG
| |
| - | GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
| |
| - | GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
| |
| - | CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA
| |
| - | ACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA
| |
| - | GTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCT
| |
| - | GCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGA
| |
| - | TCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAG
| |
| - | TTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCG
| |
| - | TAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCG
| |
| - | TTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGT
| |
| - | CAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAA
| |
| - | ACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCG
| |
| - | AGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCC
| |
| - | CAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAG
| |
| - | TGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGA
| |
| - | CTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACC
| |
| - | GGTTAA
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
| |
| - | EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
| |
| - | IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
| |
| - | CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ
| |
| - | DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG
| |
| - | QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN
| |
| - | SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/8/8d/Freiburg09_Strep%2BFluA%2BSplit%2BFokI_pMA.txt">Strep+FluA+Split+Foki_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - |
| |
| - | </div><a name="13"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> His+Dig+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/c/c7/Freiburg09_His%2BDig%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_His%2BDig%2Bmiddellinker%2BFokA_pMA.jpg" name="His+Dig+middellinker+FokA in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">His+Dig+middellinker+FokA in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGC
| |
| - | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| |
| - | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| |
| - | GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
| |
| - | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
| |
| - | CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
| |
| - | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
| |
| - | GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
| |
| - | CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
| |
| - | ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| |
| - | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| |
| - | ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
| |
| - | ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT
| |
| - | TCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA
| |
| - | CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC
| |
| - | GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC
| |
| - | ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC
| |
| - | TGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG
| |
| - | TTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCT
| |
| - | GATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACAT
| |
| - | CAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCA
| |
| - | AATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTG
| |
| - | ACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGA
| |
| - | AGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGG
| |
| - | AAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
| |
| - | SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
| |
| - | YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| |
| - | NNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR
| |
| - | ILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQA
| |
| - | DEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSN
| |
| - | GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/8/8a/Freiburg09_His%2BDig%2Bmiddellinker%2BFokA_pMA.txt">His+Dig+middellinker+FokA_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - |
| |
| - | </div><a name="14"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> His+Dig+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/3/3b/Freiburg09_His%2BDig%2Bmiddellinker%2BFokI_pMA.jpg/600px-Freiburg09_His%2BDig%2Bmiddellinker%2BFokI_pMA.jpg" name="His+Dig+middellinker+Foki in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">His+Dig+middellinker+Foki in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGC
| |
| - | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| |
| - | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| |
| - | GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
| |
| - | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
| |
| - | CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
| |
| - | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
| |
| - | GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
| |
| - | CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
| |
| - | ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| |
| - | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| |
| - | ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
| |
| - | ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT
| |
| - | TCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA
| |
| - | CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC
| |
| - | GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC
| |
| - | ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC
| |
| - | AGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG
| |
| - | TGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCT
| |
| - | GATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCT
| |
| - | GAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCA
| |
| - | AATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTG
| |
| - | ACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGA
| |
| - | GGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGG
| |
| - | AAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
| |
| - | SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
| |
| - | YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| |
| - | NNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR
| |
| - | ILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQA
| |
| - | DEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSN
| |
| - | GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/9/9f/Freiburg09_His%2BDig%2Bmiddellinker%2BFokI_pMA.txt">His+Dig+middellinker+Foki_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - | </div><a name="15"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> His+Dig+shortlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/5/58/Freiburg09_His%2BDig%2Bshortlinker%2BFokA_pMA.jpg/600px-Freiburg09_His%2BDig%2Bshortlinker%2BFokA_pMA.jpg" name="His+Dig+shortlinker+FokA in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">His+Dig+shortlinker+FokA in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGC
| |
| - | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| |
| - | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| |
| - | GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
| |
| - | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
| |
| - | CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
| |
| - | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
| |
| - | GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
| |
| - | CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
| |
| - | ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| |
| - | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| |
| - | ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
| |
| - | ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTACCGGC
| |
| - | AAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATA
| |
| - | TGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCC
| |
| - | AAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTAT
| |
| - | GGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCAT
| |
| - | TTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAG
| |
| - | CCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAG
| |
| - | CGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGA
| |
| - | ATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCG
| |
| - | TGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAAC
| |
| - | CATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGAT
| |
| - | TGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCC
| |
| - | GTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
| |
| - | SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
| |
| - | YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| |
| - | NNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEM
| |
| - | KVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQ
| |
| - | RYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVL
| |
| - | SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/d/d2/Freiburg09_His%2BDig%2Bshortlinker%2BFokA_pMA.txt">His+Dig+shortlinker+FokA_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - | </div><a name="16"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> His+Dig+shortlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/1/15/Freiburg09_His%2BDig%2Bshortlinker%2BFoki_pMA.jpg/600px-Freiburg09_His%2BDig%2Bshortlinker%2BFoki_pMA.jpg" name="His+Dig+shortlinker+Foki in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">His+Dig+shortlinker+Foki in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGC
| |
| - | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| |
| - | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| |
| - | GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
| |
| - | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
| |
| - | CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
| |
| - | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
| |
| - | GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
| |
| - | CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
| |
| - | ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| |
| - | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| |
| - | ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
| |
| - | ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTACCGGC
| |
| - | AAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATA
| |
| - | TGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCC
| |
| - | AAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTAT
| |
| - | GGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCAT
| |
| - | TTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAG
| |
| - | CGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAA
| |
| - | CGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGA
| |
| - | ATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCG
| |
| - | TGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAAC
| |
| - | CATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGAT
| |
| - | CGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCC
| |
| - | GTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
| |
| - | SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
| |
| - | YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| |
| - | NNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEM
| |
| - | KVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEME
| |
| - | RYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVL
| |
| - | SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/b/b5/Freiburg09_His%2BDig%2Bshortlinker%2BFoki_pMA.txt">His+Dig+shortlinker+Foki_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - |
| |
| - | </div><a name="17"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> His+Dig+Split+Foki_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/d/d7/Freiburg09_His%2BDig%2BSplit%2BFokI_pMA.jpg/600px-Freiburg09_His%2BDig%2BSplit%2BFokI_pMA.jpg" name="His+Dig+Split+Foki in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">His+Dig+Split+Foki in pMA Vector</p><br>
| |
| - | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243018">link to part registry</a>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG
| |
| - | CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
| |
| - | ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
| |
| - | GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
| |
| - | GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG
| |
| - | CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC
| |
| - | ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA
| |
| - | CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG
| |
| - | GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
| |
| - | GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
| |
| - | CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA
| |
| - | ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACCAGCC
| |
| - | TGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAA
| |
| - | ATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATG
| |
| - | TGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAA
| |
| - | GACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGG
| |
| - | CTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTT
| |
| - | ATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCG
| |
| - | TATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACG
| |
| - | TTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAAT
| |
| - | GGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTG
| |
| - | AGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCA
| |
| - | TATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCG
| |
| - | GTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGT
| |
| - | AAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
| |
| - | EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
| |
| - | IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
| |
| - | CKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE
| |
| - | LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKA
| |
| - | YSGGYNLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA
| |
| - | QLTRLNHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/1/11/Freiburg09_His%2BDig%2BSplit%2BFokI_pMA.txt">His+Dig+Split+Foki_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - |
| |
| - | </div><a name="18"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> His+FluA+longlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/9/9f/Freiburg09_His%2BFluA%2Blonglinker%2BFokA_pMA.jpg/600px-Freiburg09_His%2BFluA%2Blonglinker%2BFokA_pMA.jpg" name="His+FluA+longlinker+FokA in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">His+FluA+longlinker+FokA in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGC
| |
| - | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| |
| - | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| |
| - | GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
| |
| - | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
| |
| - | CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
| |
| - | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
| |
| - | TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
| |
| - | CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
| |
| - | ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| |
| - | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| |
| - | ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
| |
| - | GCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCT
| |
| - | GAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCC
| |
| - | TCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACC
| |
| - | GTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTAT
| |
| - | CGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATAC
| |
| - | CGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATA
| |
| - | GCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTAT
| |
| - | GTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATGGTG
| |
| - | GAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCG
| |
| - | GCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAA
| |
| - | ACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGG
| |
| - | TGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAAT
| |
| - | TCAACAATGGGGAGATCAATTTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
| |
| - | SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
| |
| - | YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| |
| - | NNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
| |
| - | MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRY
| |
| - | VKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSV
| |
| - | EELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/b/be/Freiburg09_His%2BFluA%2Blonglinker%2BFokA_pMA.txt">His+FluA+longlinker+FokA_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - |
| |
| - | </div><a name="19"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> His+FluA+longlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/6/6c/Freiburg09_His%2BFluA%2Blonglinker%2BFokI_pMA.jpg/600px-Freiburg09_His%2BFluA%2Blonglinker%2BFokI_pMA.jpg" name="His+FluA+longlinker+Foki in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">His+FluA+longlinker+Foki in pMA Vector</p><br>
| |
| - | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243016">link to part registry</a>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGC
| |
| - | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| |
| - | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| |
| - | GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
| |
| - | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
| |
| - | CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
| |
| - | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
| |
| - | TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
| |
| - | CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
| |
| - | ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| |
| - | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| |
| - | ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
| |
| - | GCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCT
| |
| - | GAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCC
| |
| - | TCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACC
| |
| - | GTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTAT
| |
| - | CGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATAC
| |
| - | CGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATT
| |
| - | CTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTAT
| |
| - | GTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAATGGTG
| |
| - | GAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCG
| |
| - | GCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATC
| |
| - | ACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGG
| |
| - | AGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAAT
| |
| - | TCAACAATGGCGAGATCAATTTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
| |
| - | SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
| |
| - | YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| |
| - | NNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
| |
| - | MEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERY
| |
| - | VEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSV
| |
| - | EELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/7/77/Freiburg09_His%2BFluA%2Blonglinker%2BFokI_pMA.txt">His+FluA+longlinker+Foki_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </div><a name="20"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> His+FluA+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/e/ef/Freiburg09_His%2BFluA%2BSplit%2BFokA_pMA.jpg/600px-Freiburg09_His%2BFluA%2BSplit%2BFokA_pMA.jpg" name="His+FluA+Split+FokA in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">His+FluA+Split+FokA in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCC
| |
| - | GGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTG
| |
| - | CCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCA
| |
| - | AGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAG
| |
| - | TGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAG
| |
| - | ATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACC
| |
| - | CCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGC
| |
| - | GGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAA
| |
| - | CTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACT
| |
| - | GGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCC
| |
| - | AAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCA
| |
| - | GAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACA
| |
| - | CCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATG
| |
| - | AATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA
| |
| - | CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC
| |
| - | GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC
| |
| - | ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC
| |
| - | TGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG
| |
| - | TTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCT
| |
| - | GATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACAT
| |
| - | CAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCA
| |
| - | AATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTG
| |
| - | ACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGA
| |
| - | AGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGG
| |
| - | AAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEG
| |
| - | KSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYII
| |
| - | GYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACK
| |
| - | VNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR
| |
| - | ILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQA
| |
| - | DEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSN
| |
| - | GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/5/53/Freiburg09_His%2BFluA%2BSplit%2BFokA_pMA.txt">His+FluA+Split+FokA_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - |
| |
| - |
| |
| - | </div><a name="21"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> Strep+Dig+longlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/8/8b/Freiburg09_Strep%2BDig%2Blonglinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Blonglinker%2BFokA_pMA.jpg" name="Strep+Dig+longlinker+FokA in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">Strep+Dig+longlinker+FokA in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCC
| |
| - | GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
| |
| - | CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
| |
| - | ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
| |
| - | GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
| |
| - | GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG
| |
| - | CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC
| |
| - | ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA
| |
| - | CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG
| |
| - | GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
| |
| - | GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
| |
| - | CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA
| |
| - | ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCT
| |
| - | GGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGA
| |
| - | GAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATA
| |
| - | TCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAA
| |
| - | ATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACA
| |
| - | TCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCC
| |
| - | CGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTAT
| |
| - | AACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAA
| |
| - | CCAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATC
| |
| - | CGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAA
| |
| - | GGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAA
| |
| - | TGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCA
| |
| - | AAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGG
| |
| - | GAGATCAATTTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
| |
| - | EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
| |
| - | IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
| |
| - | CKVNNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIA
| |
| - | RNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGY
| |
| - | NLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRL
| |
| - | NHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/2/22/Freiburg09_Strep%2BDig%2Blonglinker%2BFokA_pMA.txt">Strep+Dig+longlinker+FokA_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - | </div><a name="22"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> Strep+Dig+longlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/6/65/Freiburg09_Strep%2BDig%2Blonglinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Blonglinker%2BFokI_pMA.jpg" name="Strep+Dig+longlinker+Foki in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">Strep+Dig+longlinker+Foki in pMA Vector <br>
| |
| - | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243011">link to part registry</a></li>
| |
| - | </p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCC
| |
| - | GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
| |
| - | CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
| |
| - | ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
| |
| - | GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
| |
| - | GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG
| |
| - | CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC
| |
| - | ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA
| |
| - | CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG
| |
| - | GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
| |
| - | GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
| |
| - | CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA
| |
| - | ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCT
| |
| - | GGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGA
| |
| - | GAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATA
| |
| - | TCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAA
| |
| - | ATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACA
| |
| - | TCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCC
| |
| - | CGATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTAT
| |
| - | AATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAA
| |
| - | TCAGACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATC
| |
| - | CGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAA
| |
| - | GGCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAA
| |
| - | TGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCA
| |
| - | AAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGC
| |
| - | GAGATCAATTTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
| |
| - | EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
| |
| - | IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
| |
| - | CKVNNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIA
| |
| - | RNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGY
| |
| - | NLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRL
| |
| - | NHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/4/4c/Freiburg09_Strep%2BDig%2Blonglinker%2BFokI_pMA.txt">Strep+Dig+longlinker+Foki_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - |
| |
| - |
| |
| - | </div><a name="23"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> Strep+Dig+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/9/94/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokA_pMA.jpg" name="Strep+Dig+middellinker+FokA in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">Strep+Dig+middellinker+FokA in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
| |
| - | CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
| |
| - | AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
| |
| - | AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
| |
| - | GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
| |
| - | GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
| |
| - | TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
| |
| - | CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
| |
| - | AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
| |
| - | ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
| |
| - | GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
| |
| - | AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT
| |
| - | GGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAA
| |
| - | ATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAAC
| |
| - | TGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAA
| |
| - | GTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGG
| |
| - | TGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCG
| |
| - | ATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTG
| |
| - | CCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGAC
| |
| - | TCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAA
| |
| - | GCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAAC
| |
| - | TATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGC
| |
| - | CGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCG
| |
| - | GGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATC
| |
| - | AATTTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
| |
| - | TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
| |
| - | NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
| |
| - | AACKVNNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARN
| |
| - | STQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNL
| |
| - | PIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNH
| |
| - | KTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/9/9f/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokA_pMA.txt">Strep+Dig+middellinker+FokA_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - |
| |
| - |
| |
| - | </div><a name="24"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> Strep+Dig+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/7/79/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokI_pMA.jpg" name="Strep+Dig+middellinker+Foki in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">Strep+Dig+middellinker+Foki in pMA Vector<br>
| |
| - | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243012">link to part registry</a>
| |
| - | </p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
| |
| - | CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
| |
| - | AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
| |
| - | AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
| |
| - | GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
| |
| - | GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
| |
| - | TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
| |
| - | CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
| |
| - | AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
| |
| - | ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
| |
| - | GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
| |
| - | AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT
| |
| - | GGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAA
| |
| - | ATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAAC
| |
| - | TGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAA
| |
| - | GTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGG
| |
| - | TGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCG
| |
| - | ATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTG
| |
| - | CCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGAC
| |
| - | CCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAA
| |
| - | GTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAAC
| |
| - | TATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGC
| |
| - | AGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAG
| |
| - | GCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATC
| |
| - | AATTTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
| |
| - | TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
| |
| - | NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
| |
| - | AACKVNNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARN
| |
| - | STQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNL
| |
| - | PIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNH
| |
| - | ITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/7/73/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokI_pMA.txt">Strep+Dig+middellinker+Foki_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - |
| |
| - |
| |
| - | </div><a name="25"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> Strep+FluA+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/9/97/Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.jpg" name="Strep+FluA+Split+FokA in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">Strep+FluA+Split+FokA in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
| |
| - | CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
| |
| - | AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
| |
| - | AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
| |
| - | GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
| |
| - | GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
| |
| - | CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
| |
| - | CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
| |
| - | AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
| |
| - | ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
| |
| - | GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
| |
| - | AAGTGAACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAG
| |
| - | CAGAAAGTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATC
| |
| - | CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA
| |
| - | TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG
| |
| - | ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG
| |
| - | TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT
| |
| - | ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA
| |
| - | ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG
| |
| - | TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG
| |
| - | TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT
| |
| - | AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT
| |
| - | TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA
| |
| - | CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT
| |
| - | TTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
| |
| - | TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
| |
| - | NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
| |
| - | AACKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS
| |
| - | TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP
| |
| - | IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK
| |
| - | TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/7/73/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokI_pMA.txt">Strep+Dig+middellinker+Foki_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - |
| |
| - |
| |
| - | </div><a name="26"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> Strep+FluA+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/9/97/Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.jpg" name="Strep+FluA+Split+FokA in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">Strep+FluA+Split+FokA in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
| |
| - | CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
| |
| - | AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
| |
| - | AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
| |
| - | GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
| |
| - | GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
| |
| - | CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
| |
| - | CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
| |
| - | AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
| |
| - | ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
| |
| - | GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
| |
| - | AAGTGAACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAG
| |
| - | CAGAAAGTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATC
| |
| - | CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA
| |
| - | TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG
| |
| - | ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG
| |
| - | TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT
| |
| - | ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA
| |
| - | ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG
| |
| - | TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG
| |
| - | TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT
| |
| - | AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT
| |
| - | TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA
| |
| - | CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT
| |
| - | TTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
| |
| - | TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
| |
| - | NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
| |
| - | AACKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS
| |
| - | TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP
| |
| - | IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK
| |
| - | TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/6/6a/Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.txt">Strep+FluA+Split+FokA_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - |
| |
| - | </div><a name="27"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> Strep+Dig+shortlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/f/fb/Freiburg09_Strep%2BDig%2Bshortlinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Bshortlinker%2BFokA_pMA.jpg" name="Strep+Dig+shortlinker+FokA in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">Strep+Dig+shortlinker+FokA in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
| |
| - | CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
| |
| - | AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
| |
| - | AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
| |
| - | GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
| |
| - | GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
| |
| - | TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
| |
| - | CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
| |
| - | AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
| |
| - | ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
| |
| - | GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
| |
| - | AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT
| |
| - | GGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCG
| |
| - | CCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCG
| |
| - | CCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTC
| |
| - | TTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAA
| |
| - | ACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTA
| |
| - | TCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAG
| |
| - | GCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACA
| |
| - | CATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGT
| |
| - | TCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAG
| |
| - | CTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGT
| |
| - | AGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACAC
| |
| - | TGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGT
| |
| - | TAA
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
| |
| - | TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
| |
| - | NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
| |
| - | AACKVNNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQD
| |
| - | RILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQ
| |
| - | ADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNS
| |
| - | NGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/4/45/Freiburg09_Strep%2BDig%2Bshortlinker%2BFokA_pMA.txt">Strep+Dig+shortlinker+FokA_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - |
| |
| - | </div><a name="28"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> Strep+Dig+shortlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/4/4a/Freiburg09_Strep%2BDig%2Bshortlinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Bshortlinker%2BFokI_pMA.jpg" name="Strep+Dig+shortlinker+Foki in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">Strep+Dig+shortlinker+Foki in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
| |
| - | CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
| |
| - | AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
| |
| - | AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
| |
| - | GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
| |
| - | GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
| |
| - | TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
| |
| - | CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
| |
| - | AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
| |
| - | ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
| |
| - | GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
| |
| - | AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT
| |
| - | GGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCG
| |
| - | CCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCG
| |
| - | CCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTC
| |
| - | TTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAA
| |
| - | ACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTA
| |
| - | TCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAG
| |
| - | GCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACA
| |
| - | TCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGT
| |
| - | TCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAG
| |
| - | CTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGT
| |
| - | TGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTC
| |
| - | TGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGT
| |
| - | TAA
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
| |
| - | TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
| |
| - | NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
| |
| - | AACKVNNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQD
| |
| - | RILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQ
| |
| - | ADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNS
| |
| - | NGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/0/0f/Freiburg09_Strep%2BDig%2Bshortlinker%2BFokI_pMA.txt">Strep+Dig+shortlinker+Foki_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - |
| |
| - |
| |
| - | </div><a name="29"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> Strep+Dig+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/f/ff/Freiburg09_Strep%2BDig%2BSplit%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BDig%2BSplit%2BFokA_pMA.jpg" name="Strep+Dig+Split+FokA in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">Strep+Dig+Split+FokA in pMA Vector</p><br>
| |
| - | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243017">link to part registry</a>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
| |
| - | CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
| |
| - | AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
| |
| - | AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
| |
| - | GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
| |
| - | GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
| |
| - | TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
| |
| - | CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
| |
| - | AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
| |
| - | ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
| |
| - | GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
| |
| - | AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGA
| |
| - | CCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACAC
| |
| - | CGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGA
| |
| - | AATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGT
| |
| - | ACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGT
| |
| - | CTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTG
| |
| - | CCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACC
| |
| - | AAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGAT
| |
| - | GCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGA
| |
| - | ACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTG
| |
| - | TTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCT
| |
| - | GAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGC
| |
| - | TGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTT
| |
| - | CGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
| |
| - | TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
| |
| - | NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
| |
| - | AACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEY
| |
| - | IELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDT
| |
| - | KAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNY
| |
| - | KAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/9/95/Freiburg09_Strep%2BDig%2BSplit%2BFokA_pMA.txt">Strep+Dig+Split+FokA_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - |
| |
| - |
| |
| - | </div><a name="30"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> Strep+Dig+Split+Foki_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/f/fe/Freiburg09_Strep%2BDig%2BSplit%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BDig%2BSplit%2BFokI_pMA.jpg" name="Strep+Dig+Split+Foki in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">Strep+Dig+Split+Foki in pMA Vector</p><br>
| |
| - | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243015">link to part registry</a>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
| |
| - | CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
| |
| - | AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
| |
| - | AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
| |
| - | GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
| |
| - | GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
| |
| - | TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
| |
| - | CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
| |
| - | AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
| |
| - | ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
| |
| - | GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
| |
| - | AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGA
| |
| - | CCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACAC
| |
| - | CGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGA
| |
| - | AATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGT
| |
| - | ACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGT
| |
| - | CTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTG
| |
| - | CCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACA
| |
| - | AAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGAT
| |
| - | GGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGA
| |
| - | ACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTG
| |
| - | TTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCT
| |
| - | GAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGC
| |
| - | TGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTT
| |
| - | CGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
| |
| - | TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
| |
| - | NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
| |
| - | AACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEY
| |
| - | IELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVAT
| |
| - | KAYSGGYNLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNY
| |
| - | KAQLTRLNHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/6/63/Freiburg09_Strep%2BDig%2BSplit%2BFokI_pMA.txt">Strep+Dig+Split+Foki_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - |
| |
| - | </div><a name="31"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> Strep+FluA+longlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/2/22/Freiburg09_Strep%2BFluA%2Blonglinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Blonglinker%2BFokA_pMA.jpg" name="Strep+FluA+longlinker+FokA in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">Strep+FluA+longlinker+FokA in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
| |
| - | CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
| |
| - | ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
| |
| - | GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
| |
| - | GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG
| |
| - | CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC
| |
| - | GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA
| |
| - | CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG
| |
| - | GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
| |
| - | GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
| |
| - | CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA
| |
| - | ACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACC
| |
| - | GGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAA
| |
| - | ATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTA
| |
| - | CCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTC
| |
| - | TATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGC
| |
| - | CATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCA
| |
| - | AAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATG
| |
| - | CAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAA
| |
| - | CGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGT
| |
| - | TCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTG
| |
| - | AACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCT
| |
| - | GATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTC
| |
| - | GCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | GWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
| |
| - | EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
| |
| - | IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
| |
| - | CKVNNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILE
| |
| - | MKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEM
| |
| - | QRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAV
| |
| - | LSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/d/d4/Freiburg09_Strep%2BFluA%2Blonglinker%2BFokA_pMA.txt">Strep+FluA+longlinker+FokA_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - |
| |
| - | </div><a name="32"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> Strep+FluA+longlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/4/47/Freiburg09_Strep%2BFluA%2Blonglinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Blonglinker%2BFokI_pMA.jpg" name="Strep+FluA+longlinker+Foki in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">Strep+FluA+longlinker+Foki in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
| |
| - | CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
| |
| - | AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
| |
| - | AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
| |
| - | GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
| |
| - | GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
| |
| - | CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
| |
| - | CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
| |
| - | AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
| |
| - | ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
| |
| - | GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
| |
| - | AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCT
| |
| - | GGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA
| |
| - | ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA
| |
| - | ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG
| |
| - | AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGC
| |
| - | AGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGG
| |
| - | CCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGAT
| |
| - | GAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAA
| |
| - | CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAAT
| |
| - | TTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACT
| |
| - | CGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGA
| |
| - | ACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAG
| |
| - | AAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
| |
| - | TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
| |
| - | NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
| |
| - | AACKVNNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRI
| |
| - | LEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQAD
| |
| - | EMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNG
| |
| - | AVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/c/ce/Freiburg09_Strep%2BFluA%2Blonglinker%2BFokI_pMA.txt">Strep+FluA+longlinker+Foki_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - |
| |
| - | </div><a name="33"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> Strep+FluA+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/d/d8/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokA_pMA.jpg" name="Strep+FluA+middellinker+FokA in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">Strep+FluA+middellinker+FokA in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
| |
| - | CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
| |
| - | AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
| |
| - | AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
| |
| - | GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
| |
| - | GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
| |
| - | CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
| |
| - | CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
| |
| - | AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
| |
| - | ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
| |
| - | GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
| |
| - | AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAA
| |
| - | TCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGT
| |
| - | GCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAG
| |
| - | ACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGC
| |
| - | TATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTA
| |
| - | TACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCT
| |
| - | ATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGT
| |
| - | TATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATG
| |
| - | GTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGA
| |
| - | GCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCAT
| |
| - | AAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGG
| |
| - | CGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTA
| |
| - | AATTCAACAATGGGGAGATCAATTTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
| |
| - | TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
| |
| - | NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
| |
| - | AACKVNNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMK
| |
| - | VMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
| |
| - | YVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLS
| |
| - | VEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/4/48/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokA_pMA.txt">Strep+FluA+middellinker+FokA_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - |
| |
| - | </div><a name="34"></a>
| |
| - | <h3 class="art-PostHeaderIcon-wrapper"> Strep+FluA+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
| |
| - | </div>
| |
| - | <div class="art-PostContent">
| |
| - | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| - |
| |
| - |
| |
| - | <table border="5">
| |
| - | <tr>
| |
| - | <td align="center">
| |
| - | <img src="https://static.igem.org/mediawiki/2009/thumb/b/b9/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokI_pMA.jpg" name="Strep+FluA+middellinker+Foki in pMA Vector" width="480" height="480" />
| |
| - | <br><p align="center">Strep+FluA+middellinker+Foki in pMA Vector</p>
| |
| - | </td>
| |
| - | <td align="left">
| |
| - | nucleotid sequence of ORF-1:<br>
| |
| - |
| |
| - | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
| |
| - | CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
| |
| - | AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
| |
| - | AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
| |
| - | GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
| |
| - | GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
| |
| - | CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
| |
| - | CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
| |
| - | AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
| |
| - | ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
| |
| - | GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
| |
| - | AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAA
| |
| - | TCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGT
| |
| - | GCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAG
| |
| - | ACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGC
| |
| - | TATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTA
| |
| - | TACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCGT
| |
| - | ATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACGT
| |
| - | TATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAATG
| |
| - | GTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGA
| |
| - | GCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCAT
| |
| - | ATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGG
| |
| - | TGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTA
| |
| - | AATTCAACAATGGCGAGATCAATTTTACCGGTTAA
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <h4>AA sequence of ORF-1:<br></h4>
| |
| - | <table border="0">
| |
| - | <p align="justify"><td align="left">
| |
| - | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
| |
| - | TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
| |
| - | NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
| |
| - | AACKVNNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMK
| |
| - | VMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMER
| |
| - | YVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLS
| |
| - | VEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| - |
| |
| - |
| |
| - |
| |
| - | <br><br></p></td></table>
| |
| - |
| |
| - | <h4>Commented GenBank file</h4>
| |
| - | <li> <a href="https://static.igem.org/mediawiki/2009/a/a3/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokI_pMA.txt">Strep+FluA+middellinker+Foki_pMA.txt</a></li>
| |
| - | <br>
| |
| - | <br>
| |
| - | <br>
| |
| | | | |
| | | | |
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