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Team:Imperial College London/M3/Genetic/DpnTaq
From 2009.igem.org
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DpnII comes from Streptococccus Pneumonia. <br> | DpnII comes from Streptococccus Pneumonia. <br> | ||
<br> | <br> | ||
| - | + | DpnII recognises the sequence GATC and cleave before G. <br> | |
| - | Cells of Streptococccus Pneumonia contain either DpnII, or the more well-known DpnI as part of their restriction machinery. Both recognise the same sequence of GATC | + | <br> |
| + | Cells of Streptococccus Pneumonia contain either DpnII, or the more well-known DpnI as part of their restriction machinery. Both recognise the same sequence of GATC. However, DpnII only acts on the unmethylated strand, as opposed to DpnI that only cleaves methylated strands. <br> | ||
<br> | <br> | ||
The DpnII restriction cassette contains 2 methylases and 1 endonuclease.<br> | The DpnII restriction cassette contains 2 methylases and 1 endonuclease.<br> | ||
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TaqI comes from the thermophile thermus aquaticus. <br> | TaqI comes from the thermophile thermus aquaticus. <br> | ||
<br> | <br> | ||
| - | |||
TaqI recognises the sequence TCGA and cleaves after T. <br> | TaqI recognises the sequence TCGA and cleaves after T. <br> | ||
<br> | <br> | ||
| - | Interestingly, the TaqI gene contains 7 TaqI sites, probably important in | + | Interestingly, the TaqI gene contains 7 TaqI sites, probably important in autoregulation of TaqI expression. <br> |
<br> | <br> | ||
The TaqI restriction cassette contains 1 methylase and 1 endonuclease. <br> | The TaqI restriction cassette contains 1 methylase and 1 endonuclease. <br> | ||
Revision as of 21:50, 17 October 2009
DpnII
DpnII comes from Streptococccus Pneumonia.
DpnII recognises the sequence GATC and cleave before G.
Cells of Streptococccus Pneumonia contain either DpnII, or the more well-known DpnI as part of their restriction machinery. Both recognise the same sequence of GATC. However, DpnII only acts on the unmethylated strand, as opposed to DpnI that only cleaves methylated strands.
The DpnII restriction cassette contains 2 methylases and 1 endonuclease.
TaqI
TaqI comes from the thermophile thermus aquaticus.
TaqI recognises the sequence TCGA and cleaves after T.
Interestingly, the TaqI gene contains 7 TaqI sites, probably important in autoregulation of TaqI expression.
The TaqI restriction cassette contains 1 methylase and 1 endonuclease.
References
