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Lab Sep 22 2009
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Jasoncummer (Talk | contribs) (New page: Alright, the plan for today is to do the assemblies K19, 20 and 21. Rehydrate the new ribokey J23070, and retransform R0051, E0240, C0012 and K115017. 7pm - Assembled K235019, 20, 21...) |
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I can come in tomorrow afternoon at 4.30 to start broth cultures for these. (Reminder to autoclave epis! They will be needed for the miniprep the next day!) And on Thursday I can miniprep these at 8.30am. If no one else can be here, I can run a gel at 2.30 on Thurs but it would be great if anyone else can do it that day. | I can come in tomorrow afternoon at 4.30 to start broth cultures for these. (Reminder to autoclave epis! They will be needed for the miniprep the next day!) And on Thursday I can miniprep these at 8.30am. If no one else can be here, I can run a gel at 2.30 on Thurs but it would be great if anyone else can do it that day. | ||
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| + | Back to [https://2009.igem.org/Team:VictoriaBC/Labnotebook LAB NOTEBOOK] | ||
Latest revision as of 22:11, 17 October 2009
Alright, the plan for today is to do the assemblies K19, 20 and 21. Rehydrate the new ribokey J23070, and retransform R0051, E0240, C0012 and K115017.
7pm
- Assembled K235019, 20, 21.
- Rehydrated J23070 (new ribokey), J04630 (GFP + stop: to use as a control)
- Transformed all of the above
- Plated all of the above plus new streak of R0051 stab (I'm not sure what kind of growth to expect from that.)
I can come in tomorrow afternoon at 4.30 to start broth cultures for these. (Reminder to autoclave epis! They will be needed for the miniprep the next day!) And on Thursday I can miniprep these at 8.30am. If no one else can be here, I can run a gel at 2.30 on Thurs but it would be great if anyone else can do it that day.
Back to LAB NOTEBOOK
