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Team:UNIPV-Pavia/Methods Materials
From 2009.igem.org
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| + | <h1>Protocols</h1> | ||
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| + | <a href="https://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/Lb"> LB medium preparation </a> | ||
| + | *[[Team:UNIPV-Pavia/Methods_Materials/Lb|Lb medium]] | ||
| + | *[[Team:UNIPV-Pavia/Protocols/Transformation|Transformation]] | ||
| + | *[[Team:UNIPV-Pavia/Protocols/PlasmidExtraction|Plasmid extraction]] | ||
| + | *[[Team:UNIPV-Pavia/Protocols/Digestion|BioBrick digestion with restriction enzymes]] | ||
| + | *[[Team:UNIPV-Pavia/Protocols/GelExtraction|DNA gel extraction]] | ||
| + | *[[Team:UNIPV-Pavia/Protocols/Precipitation|DNA precipitation with sodium acetate]] | ||
| + | *[[Team:UNIPV-Pavia/Protocols/AntarcticPhosphatase|Antarctic Phosphatase]] | ||
| + | *[[Team:UNIPV-Pavia/Protocols/Ligation|Ligation]] | ||
| + | *[[Team:UNIPV-Pavia/Protocols/Pcr|PCR]] | ||
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| + | <br> | ||
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'''Gycerol stock''' | '''Gycerol stock''' | ||
*Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol | *Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol | ||
Revision as of 14:39, 22 June 2009
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Protocols<a href="https://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/Lb"> LB medium preparation </a>
Gycerol stock
X-Gal Staining Protocol for beta Galactosidase (blue/white screening) The principle is that X-Gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside) turns blue when reacts with beta-galactosidase.
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