September/26 September 2009

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(New page: 1.colony check<br> sample no no of colony 37 0 54 + 55 + <br> 2.min prep<br> result of nano drop sample no. conc. X5 121 ng/uL ...)
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Revision as of 05:48, 18 October 2009

1.colony check

sample no   no of colony
  37            0
  54            +
  55            +

2.min prep
result of nano drop

sample no.   conc.
  X5         121 ng/uL
  X6         121
  X7         209
  X8         189
 2-18B       137
  53         7.8

3.ligation check

K204036

Vector		Insert
1-1F	3	1-12M	10
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	12	dH₂O	6
total	20uL	total	20uL

K204037

Vector		Insert
1-1F	3	1-8K	5
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	12	dH₂O	11
total	20uL	total	20uL

K204038

Vector		Insert
1-1F	1	1-8I	16
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	15	dH₂O	0
total	20uL	total	20uL

K204039

Vector		Insert
1-1F	3	2-8E	8
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	12	dH₂O	8
total	20uL	total	20uL

K204053

Vector		Insert
1-1F	3	33	12
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	12	dH₂O	4
total	20uL	total	20uL

↓
37degree , 3hr
Agarose Gel Electro-phoresis
↓
gel cut
purification
ligation

4.transformation

sample no.
55 , X4-A , 33 , 1-12E