Team:HKUST/Ref1

References

[1]R.J. Bryson-Richardson, D.W. Logan, P.D. Currie and I.J. Jackson, 2004. Large-scale analysis of gene structure in rhodopsin-like GPCRs: evidence for widespread loss of an ancient intron, Gene 338 (2004), pp. 15–23.

[2]E. Jacoby, R. Bouhelal, M. Gerspacher and K. Seuwen, The 7 TM G-protein-coupled receptor target family, ChemMedChem 1 (2006), pp. 761–782.

[3]Hinako Suga, Tatsuya Haga, 2007. Ligand screening system using fusion proteins of G protein-coupled receptors with G protein α subunits. Neurochemistry International, Volume 51, Issues 2-4, July-September 2007, Pages 140-164

[4]Sato K. and Touhara K. (2008) Insect olfaction: receptors, signal transduction, and behavior. Results Probl. Cell Differ. Epub ahead of print.

[5]Versele, M. et al. (2001) Sex and sugar in yeast: two distinct GPCR systems. EMBO Rep. 2, 574–579

[6]Graham Ladds, Alan Goddard, John Davey,2005. Functional analysis of heterologous GPCR signalling pathways in yeast.Trends in Biotechnology, Volume 23, Issue 7, July 2005, Pages 367-373

[7]Johnson GL, Dhanasekaran N. The G-protein family and their interaction with receptors. Endocr Rev. 1989; 10: 317–331.

[8]J. Minic, M.A. Persuy, E. Godel, J. Aioun, I. Connerton, R. Salesse and E. Pajot-Augy, Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening, FEBS J. 272 (2005), pp. 524–537.

[9]Minic, J., Sautel, M., Salesse, R., and Pajot-Augy, E. (2005) Yeast System as a Screening Tool for Pharmacological Assessment of G Protein Coupled Receptors .Curr. Med. Chem. 12, 961-969
[10]Radhika, V. et al . 2007. Chemical sensing of DNT by engineered olfactory yeast strain. Nat. Chem. Biol. 3 : 325–330

[11]Crowe ML, Perry BN & Connerton IF (2000) Golf complements a GPA1 null mutation in Saccharomyces cerevisiae and functionally couples to the STE2 pheromone receptor. J Recept Signal Transduct Res 20, 61–73.

[12]Janke et al., 2004 C. Janke, M.M. Magiera, N. Rathfelder, C. Taxis, S. Reber, H. Maekawa, A. Moreno-Borchart, G. Doenges, E. Schwob, E. Schiebel and M. Knop, A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes, Yeast 21 (2004), pp. 947–962.

[13]Zhang Y., Chou J.H., Bradley J., Bargmann C.I., Zinn K.(1997) The Caenorhabditis elegans seven-transmembrane protein ODR-10 functions as an odorant receptor in mammalian cells. Proc. Natl. Acad. Sci. 94:12162–12167.

Background

Experimental Design

Parts Design

Experimental Results

Future Work

Reference