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Team:DTU Denmark/modelling
From 2009.igem.org
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Here a model for the GFP expression under the control of “The Redoxilator” expression system is derived. The model links the level of the signal molecules NADH and NAD to the final expression of GFP through the events of transcription factor activation, promoter activation, synthesis and degradation of mRNA and finally synthesis, dilution and degradation of GFP. | Here a model for the GFP expression under the control of “The Redoxilator” expression system is derived. The model links the level of the signal molecules NADH and NAD to the final expression of GFP through the events of transcription factor activation, promoter activation, synthesis and degradation of mRNA and finally synthesis, dilution and degradation of GFP. | ||
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| + | Abbreviations: | ||
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| + | <i>TF</i>= the transcription factor based on the REX protein. <br> | ||
| + | <i>TF'</i>= active transcription factor. <br> | ||
| + | <i>N</i>= NAD <br> | ||
| + | <i>H</i>= NADH <br> | ||
| + | <i>TFH</i>= the transcription factor bound to NADH. <br> | ||
| + | <i>TFN</i>= the transcription factor bound to NAD. <br> | ||
| + | <i>P</i>= Promoter upstream of GFP <br> | ||
| + | <i>P'</i> = activated/transcribed promoter <br> | ||
| + | <i>tf</i>= fraction of active transcription factor <br> | ||
| + | <i>Pf</i>= fraction of active promoter<br> | ||
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<p align="justify">Modelling of the redoxilator is in progress...</p> | <p align="justify">Modelling of the redoxilator is in progress...</p> | ||
Revision as of 09:17, 18 October 2009
| Home | The Team | The Project | Parts submitted | Modelling | Notebook |
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Also check out our Danish website - DTU iGEM 2009 |
Modelling of GFP expression Introduction Here a model for the GFP expression under the control of “The Redoxilator” expression system is derived. The model links the level of the signal molecules NADH and NAD to the final expression of GFP through the events of transcription factor activation, promoter activation, synthesis and degradation of mRNA and finally synthesis, dilution and degradation of GFP. Abbreviations: TF= the transcription factor based on the REX protein. TF'= active transcription factor. N= NAD H= NADH TFH= the transcription factor bound to NADH. TFN= the transcription factor bound to NAD. P= Promoter upstream of GFP P' = activated/transcribed promoter tf= fraction of active transcription factor Pf= fraction of active promoter Modelling of the redoxilator is in progress... |
Modelling facts Modelling is done using MATLAB with simbiology |
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