Team:Wash U/Calendar-Home/10 June 2009

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== Wednesday June 10, 2009 ==
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'''Altering Transformation Protocol to adhere to iGem Help Page'''
Estimated 5-15ng of DNA per well.
Estimated 5-15ng of DNA per well.

Revision as of 17:50, 22 June 2009



Wednesday June 10, 2009

Altering Transformation Protocol to adhere to iGem Help Page

Estimated 5-15ng of DNA per well.

Using 5ul of DNA (diluted 1:16 in TE)

Allow the DNA and competent cells to sit on ice for 30 minutes

Heat shock at 42ºC for 60 sec in water bath.

Recover on ice for 5 min.

Incubate at 37ºC for 2 hr while the tubes are rotating.

Plate 250µL on an LB plate with the appropriate antibiotic.


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