Team:DTU Denmark/modelling

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Modelling of GFP expression Introduction Here a model for the GFP expression under the control of “The Redoxilator” expression system is derived. The model links the level of the signal molecules NADH and NAD to the final expression of GFP through the events of transcription factor activation, promoter activation, synthesis and degradation of mRNA and finally synthesis, dilution and degradation of GFP. Abbreviations TF = the transcription factor based on the REX protein TF' = active transcription factor N = concentration of NAD H = concentration of NADH TFH = concentration of the transcription factor bound to NADH. TFN = concentration of the transcription factor bound to NAD. P = Promoter upstream of GFP P' = activated/transcribed promoter tf = fraction of active transcription factor Pf = fraction of active promoter NADH and NAD binding to the transcription factor XX Figure of TF binding to NADH and NAD Both NADH and NAD binds reversible to the same site of the transcription factor (TF): Dissociation of TF and NADH XX insert here at equilibrium: Dissociation of TF and NAD:

Modelling facts Modelling is done using MATLAB with simbiology

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