Team:Paris/Addressing testing

From 2009.igem.org

(Difference between revisions)
(→Export system)
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== Export system ==
== Export system ==
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We finally thought that it won't be neccesary to overexpress the Tat system, nevertheless we have run a few experiments before starting to focus on others parts of the project.
-
 
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Time required : A week.
-
Time required : A week !
+

Revision as of 14:26, 18 October 2009

iGEM > Paris > WetLab > Addressing

WetLab

Main - Addressing - Production - reception -
Addressing


Adressing system

global constructions :


plasmid = PSB3T5


Time required : A lOT !!!!!



Experiments ran :

column 1 column 2 column 3 column 4
PCR :

ClyA Cterm matrix : ? Oligo : TM :

ClyA Nterm matrix : ? Oligo : TM :

mRFP Cterm matrix : ? Oligo : TM :

mRFP Nterm plasmid : ? Oligo : TM :

PCR :

-

PCR :

-

PCR :

-

Verification on gel :

ok

Verification on gel :

-

Verification on gel :

-

Verification on gel :

-

Purification on gel :

ok

Purification on gel :

-

Purification on gel :

-

Purification on gel :

-

digestion:

ClyA Cterm S/P

RFP Nterm X/P

PBAD vector : ? E/S


digestion:

ClyA Cter-RFP Nter vector PSB1A3 E/X

digestion:

-

digestion:

-

verification digestion:

ok

verification digestion:

ok

verification digestion:

-

verification digestion:

-


ligation:

ClyA Cter-RFP Nter vector PSB1A3

ligation:

PBAD E/S ClyA Cter-RFP Nter PSB1A3 E/X (x2) PBAD S/P ClyA Cter-RFP Nter PSB? X/P (x2)

ligation:

-

ligation:

-

PCR colo :

ok

PCR colo :

ok

PCR colo :

-

PCR colo :

-


miniprep:

clone :

miniprep:

clone :

miniprep:

-

miniprep:

-


sequencing :

ok

sequencing :

ok

sequencing :

-

sequencing :

-

stock glycerol:

S? S?

stock glycerol

S?

stock glycerol

-

stock glycerol

-



Export system

We finally thought that it won't be neccesary to overexpress the Tat system, nevertheless we have run a few experiments before starting to focus on others parts of the project.


Time required : A week.



Experiments ran :

column 1 column 2 column 3 column 4


PCR :

TatABCE matrix :

PCR : PCR : PCR :
Verification on gel :

ok

Verification on gel :

-

Verification on gel :

-

Verification on gel :

-

Purification on gel :

ok

Purification on gel :

-

Purification on gel :

-

Purification on gel :

-


digestion:

TatABCE

digestion: digestion: digestion:
verification digestion:

ok

verification digestion:

-

verification digestion:

-

verification digestion:

-


ligation:

TatABCE

ligation: ligation: ligation:
PCR colo :

ok

PCR colo :

-

PCR colo :

-

PCR colo :

-

miniprep:

STOPPED

miniprep:

-

miniprep:

-

miniprep:

-

sequencing :

-

sequencing :

-

sequencing :

-

sequencing :

-


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