Team:Tsinghua/Result3
From 2009.igem.org
(Difference between revisions)
GuoQiangChen (Talk | contribs) (→Targeted Biobrick Construction) |
GuoQiangChen (Talk | contribs) (→Targeted Biobrick Construction) |
||
Line 1: | Line 1: | ||
=Targeted Biobrick Construction= | =Targeted Biobrick Construction= | ||
- | In this section, we were contructing two contructs in paralell. First, standardized biobrick for parts registry, where Targeted Biobrick can be served for further use by other teams or researchers. Second, incorporate Targeted Biobrick into an expression vector pET-28a to test its expression. | + | In this section, we were contructing two contructs in paralell. First, standardized biobrick for parts registry, where Targeted Biobrick can be served for further use by other teams or researchers. Second, incorporate Targeted Biobrick into an expression vector pET-28a to test its expression. Targeted Biobrick was contructed via two rounds of PCR. |
[[Image:j42.png|600px|center|j42|thumb|Synthesis of Targeted Biobrick. A.pAdEasy-1 plasmid extraction; B. First round PCR to modify fiber gene; C.Second round PCR to modify fiber gene.]] | [[Image:j42.png|600px|center|j42|thumb|Synthesis of Targeted Biobrick. A.pAdEasy-1 plasmid extraction; B. First round PCR to modify fiber gene; C.Second round PCR to modify fiber gene.]] |
Revision as of 19:22, 18 October 2009
Targeted Biobrick Construction
In this section, we were contructing two contructs in paralell. First, standardized biobrick for parts registry, where Targeted Biobrick can be served for further use by other teams or researchers. Second, incorporate Targeted Biobrick into an expression vector pET-28a to test its expression. Targeted Biobrick was contructed via two rounds of PCR.