Team:Tsinghua

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Since a significant procedure in gene therapy is to construct vectors to infect target cells and deliver cure gene into them,efficent vectors play a big role. And until now researchers using adenoviruses did a good job, but the production of virus suffers from high cost and low output. This is why we attempt to build a highly productive carrier in the bacteria. We transform the structure genes of the phage into the bacteria with specific chimera genes attached to the structural genes. We attempt to engineer lambda phage so that it has similar tranfection function as adenoviruses, taking advantage of their similar structures. We attach the engineered fiber from adenoviruses to phages to achieve better transfection efficiency and specificity. At the same time, we use the cosmid in the phage to carry the cure gene. As a result

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-           <td height="191" colspan="2" align="left" background="http://www.tsinghua.edu.cn/cic_jsp/qhdwzy/index_images/index_21.gif"><div id="overview">&nbsp; &nbsp;As we know, a relatively significant procedure in gene therapy is to construct a vector to infect target cells and deliver cure gene into them. As a result, the vectors act as a big role. And till now researchers use Adenoviruses did a good job, but the problems of high cost and low production of the virus have been not solved. This is why we attempt to build a highly production carrier in the bacteria. We transform the structure genes of the phage into the bacteria with specific chimera genes attach to the structural genes. We attempt to simulate the Adenoviruses by the phage, for they share the similar structure. And we attach the fiber to the phage to enhance the transformation efficiency. <!--Not only we use the cosmid in the phage to carry the cure gene, which has great capability to carry large and multiple genes, but also the cure genes are tissue specific.--></div></td>
+           <td height="191" colspan="2" align="left" background="http://www.tsinghua.edu.cn/cic_jsp/qhdwzy/index_images/index_21.gif"><div id="overview">&nbsp; &nbsp;Since a significant procedure in gene therapy is to construct vectors to infect target cells and deliver cure gene into them,efficent vectors play a big role. And until now researchers using adenoviruses did a good job, but the production of virus suffers from high cost and low output. This is why we attempt to build a highly productive carrier in the bacteria. We transform the structure genes of the phage into the bacteria with specific chimera genes attached to the structural genes. We attempt to engineer lambda phage so that it has similar tranfection function as adenoviruses, taking advantage of their similar structures. We attach the engineered fiber from adenoviruses to phages to achieve better transfection efficiency and specificity. At the same time, we use the cosmid in the phage to carry the cure gene. As a result <!--The engineered phage vectors not only have great capability to carry large and multiple genes, but also can tissue specific transfection.--></div></td>
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