IBB Pune/1 October 2009

From 2009.igem.org

(Difference between revisions)
(New page: We performed more restriction digestions and ligations today. Ak3-EP TorAGFP-XP YcdGFP-XP)
 
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We performed more restriction digestions and ligations today.
We performed more restriction digestions and ligations today.
 +
Digestions:
 +
{Promoter-RBS} -ES
Ak3-EP
Ak3-EP
-
TorAGFP-XP
+
TorA-GFP-XP
-
YcdGFP-XP
+
YcdB-GFP-XP
 +
 
 +
 
 +
Ligations:
 +
 
 +
{Promoter-RBS}-YcdB-GFP (in AK3)
 +
 
 +
{Promoter-RBS}-TorA-GFP (in AK3)
 +
 
 +
 
 +
Transformations:
 +
 
 +
We transformed the ligations of the previous two days.

Latest revision as of 11:28, 19 October 2009

We performed more restriction digestions and ligations today.

Digestions:

{Promoter-RBS} -ES

Ak3-EP

TorA-GFP-XP

YcdB-GFP-XP


Ligations:

{Promoter-RBS}-YcdB-GFP (in AK3)

{Promoter-RBS}-TorA-GFP (in AK3)


Transformations:

We transformed the ligations of the previous two days.