Team:KULeuven/28 September 2009

From 2009.igem.org

(Difference between revisions)
Deepstar (Talk | contribs)
(New page: {{Team:KULeuven/Common2/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Notebook}} {{Team:KULeuven/Common2/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don...)
Newer edit →

Revision as of 11:36, 19 October 2009

Project progress

Progress of parts

[edit] Blue Light Receptor

re enting of the cultures made on sunday (27/09)

[edit] Vanillin Production

  • Miniprep of PEFT, PAMSCO electroporation, PAMSCOEFT electroporation
part concentration 260/280 260/230
PEFT1 240,3
PEFT2 169,6
PAMSCO1 184,3
PAMSCO2 184,5
PAMSCOT1 232,5
PAMSCOT2 176,6
PAMSCOT3 293,8
PAMSCOT4 123,1
PAMSCOEFT1 87,4
PAMSCOEFT2 206,9
PAMSCOEFT3 218,4
PAMSCOEFT4 188,2
  • Restriction digest with E/S
  • PAMSCOT has the right length!
  • PAMSCOEFT appears to be a self-closed Terminator vector
  • Transfered an additional 6 colonies from PAMSCOEFT to another plate and liquid cultures

[edit] Vanillin Receptor

[edit] Key/Lock/Anti-Key