Team:Freiburg bioware/cloning1

From 2009.igem.org

(Difference between revisions)
Line 120: Line 120:
</a><br>
</a><br>
*<a href="#8">HisTag_pMA
*<a href="#8">HisTag_pMA
 +
</a><br>
 +
*<a href="#9">shortlinker26_pMA
 +
</a><br>
 +
*<a href="#10">Middellinker38_pMA
 +
</a><br>
 +
*<a href="#11">LongLinkerA50_pMA
</a><br>
</a><br>
Line 490: Line 496:
-
<a name="8"></a>
+
<a name="9"></a>
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;shortlinker26_pMA <span class="art-PostHeader"></span> </h3>
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;shortlinker26_pMA <span class="art-PostHeader"></span> </h3>
<div class="art-PostContent">
<div class="art-PostContent">
Line 527: Line 533:
-
<a name="8"></a>
+
<a name="10"></a>
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Middellinker38_pMA <span class="art-PostHeader"></span> </h3>
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Middellinker38_pMA <span class="art-PostHeader"></span> </h3>
<div class="art-PostContent">
<div class="art-PostContent">
Line 563: Line 569:
-
<a name="8"></a>
+
<a name="11"></a>
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;LongLinkerA50_pMA <span class="art-PostHeader"></span> </h3>
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;LongLinkerA50_pMA <span class="art-PostHeader"></span> </h3>
<div class="art-PostContent">
<div class="art-PostContent">

Revision as of 13:24, 19 October 2009

FREiGEM

 Index



 Parts

 FokA_pMA

back to index

FokA in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGA TGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTG ATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGAT GAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAA CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAAT TCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACC CGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGA GCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAG AAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHL GGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNE WWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTL TLEEVRRKFNNGEINFTG

    Commented GenBank file

  • FokA_pMA.txt



    •  Foki_pMA

    back to index

    Foki in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGC AGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGG CCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGAT GAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAA CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAAT TTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACT CGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGA ACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAG AAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHL GGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQTRNKHLNPNE WWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLIGGEMIKAGTL TLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Foki_pMA.txt



    •  Dig_pMA

    back to index

    Dig in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTGAAGCCCGT GGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGCAGGTGGCCG CTTATCCCGACCACATCACCAAGTACGGCAAGTGCGGCTGGGCCGAGTAC ACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTACAGCGTGATCCACGG CAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTGTGGGCGACAGCAAGA TCGGCAAGATCTACCACAGCTACACCATCGGCGGCGTGACCCAGGTGGGC GTGAGCAACGTGCTGTCCACCGACAACAAGAACTACATCATCGGCTACTT TTGCAGATACGACGAGGACAAGAAGGGCCACTGGGACGCCGTGTGGGTGC TGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAAC TACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGA CTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCAACTGGTCCCACCCCC AGTTCGAAAAGACCGGTTAA

    AA sequence of ORF-1:

    MAGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGKSVKVS RYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIGYFCRY DEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKVNNSNW SHPQFEKTG

    Commented GenBank file

  • Dig_pMA.txt



    •  FluA_pMA

    back to index

    FluA in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTGAAGCCCGT GGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGGAGGTGGCCA AGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGCGGCTGGGCCGAGTAC ACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATACGACGTGATCCACGG CAAAGAATACTTCATGGAAGGCACCGCCTACCCCGTGGGCGACAGCAAGA TCGGCAAGATCTACCACAGCCGGACCGTGGGCGGCTACACCAGAAAGACC GTGTTCAACGTGCTGTCCACCGACAACAAGAACTACATCATCGGCTACAG CTGCCGCTACGACGAGGACAAGAAGGGCCACTGGGACCACGTGTGGGTGC TGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAAC TACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGA CTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCGGTTAA

    AA sequence of ORF-1:

    MAGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGKSVKVS RYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIGYSCRY DEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKVNNTG

    Commented GenBank file

  • FluA_pMA.txt



    •  DsbA_pMA

    back to index

    DsbA in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC ATCGGCGACCGGTTA

    AA sequence of ORF-1:

    MKKIWLALAGLVLAFSASATG

    Commented GenBank file

  • DsbA_pMA.txt



    •  StrepTag_pMA (iGEM team Freiburg 08)

    back to index

    StrepTag in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGTT

    AA sequence of ORF-1:

    MAGWSHPQFEKTG

    Commented GenBank file

  • StrepTag_pMA.txt



    •  HisTag_pMA (iGEM team Freiburg 08)

    back to index

    HisTag in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    GCCGGCCATCATCATCATCATCATACCGGT

    AA sequence of ORF-1:

    AGHHHHHHTG

    Commented GenBank file

  • HisTag_pMA.txt



    •  shortlinker26_pMA

    back to index

    shortlinker26 in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGGTGGTTCTGGTACCGGT

    AA sequence of ORF-1:

    MAGGGSGTG

    Commented GenBank file

  • shortlinker26_pMA.txt



    •  Middellinker38_pMA

    back to index

    Middellinker38 in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGT

    AA sequence of ORF-1:

    MAGGGSGGGSGTG

    Commented GenBank file

  • Middellinker38_pMA.txt



    •  LongLinkerA50_pMA

    back to index

    LongLinkerA50 in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGT

    AA sequence of ORF-1:

    MAGGGSGGGSGTG

    Commented GenBank file

  • LongLinkerA50_pMA.txt





    • contact:  freigem09@googlemail.com

    Retrieved from "http://2009.igem.org/Team:Freiburg_bioware/cloning1"