Team:Paris/Freezer PCR products

From 2009.igem.org

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{{Template:Paris2009}}
{{Template:Paris2009}}
{{Template:Paris2009_menu}}
{{Template:Paris2009_menu}}
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==Freezer==
==Freezer==
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<html>
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<style type="text/css">
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#left-side {
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    position: absolute;
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    height: 23px;
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    width: 30px;
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    top: 0px;
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    left: 10px;
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    margin-top:10px;
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    padding-top: 7px;
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    background: url(https://static.igem.org/mediawiki/2009/1/1b/Left_menu_pari.png);
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    z-index:4;
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}
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#middle-side {
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    height: 25px;
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    width: 650px;
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    position: absolute;
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    top: 0px;
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    left: 30px;
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    margin-top:10px;
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    padding-top: 5px;
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    background: #dadada;
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    z-index:5;
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}
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#right-side {
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    position: absolute;
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    height: 23px;
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    width: 30px;
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    margin-top:10px;
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    padding-top: 7px;
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    top: 0px;
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    left: 670px;
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    background: url(https://static.igem.org/mediawiki/2009/4/40/Right_menu_paris.png);
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    z-index:4;
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}
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a.menu_sub {
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    padding-left: 7px;
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    padding-right: 7px;
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}
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a.menu_sub_active {
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    padding-left: 7px;
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    padding-right: 7px;
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    color:#b0310e;
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    font-weight:bold;
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}
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</style>
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<div id="left-side"></div>
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<div id="middle-side"><center>
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer#bottom"> Main </a>|
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<a class="menu_sub_active" href="https://2009.igem.org/Team:Paris/Freezer_PCR_products#bottom"> PCR Products</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Plasmids#bottom"> Plasmids</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Digestion_Products#bottom"> Digestion Products</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#bottom"> Strains (Glycerol stock)</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/constructions#bottom"> Constructions in progress</a>
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</center>
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</div>
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<div id="right-side"></div>
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</html>
<center>
<center>
[[Team:Paris/Freezer_Primers#Freezer | Primers]] -  
[[Team:Paris/Freezer_Primers#Freezer | Primers]] -  

Revision as of 15:21, 19 October 2009

iGEM > Paris > Freezer > PCR Products


Freezer

Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock) - Construction in progress


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol

PCR products

PCR products
Ref Name Oligo Fw Oligo Rev Tm used(Tm cal.) (°C) Matrix Length (bp) Comments Quantity [ ] (ng/µL) Date
A1 Tg3p_fusion N term O18 O17  ? (63.5)  ? 274 Amplification of N-term domain of M13's g3p  ?  ? 08/05
A2 TE3_fusion N term O15 O16  ?  ??? 1036 Amplification of N-term domain of colicin E3  ?  ? 08/05
A3 clyA_fusion C term O31 O10  ?(55.5) F4 987 Amplification of clyA with RBS in the primer  ?  ? 08/05
A4 OmpAsignal_fusion C term O11 O12  ?(70.4) F4 135 Amplification of OmpA signal for export to periplasm  ?  ? 08/05
A5 tolRII_fusion N term O13 O1 (73.5) F4 279 Amplification of tolR  ?  ? 08/05
A6 tatABC_RBS O14 O15 (63.5) F4 1634 Amplification of tatA with RBS  ?  ? 08/05
A7 g3p_std O19 O20 (52) P12 1287 For standardisation of g3p and adding SacI site  ?  ? 08/08
A8 OmpA_L_std O21 O22 (59.5) P6 525 For standardisation of OmpA linker (BBaK103006)  ?  ?  ?
A9 PFec A O29 O30 (65.1)  ? 163 For signal transduction  ?  ? 08/07
A10 clyA_fusion C term O31 O10  ?(55.5) F4 987 Amplification of clyA with RBS in the primer  ?  ?  ?
A11 OmpAsignal_fusion C term O11 O12  ?(70.4) F4 135 Amplification of OmpA signal for export to periplasm  ?  ?  ?
A12 Tg3p_fusion N term O18 O17  ? (63.5)  ? 274 Amplification of N-term domain of M13's g3p  ?  ? 08/07
A13 tolRII_fusion N term O13 O1 (73.5) F4 279 Amplification of tolR  ?  ?  ?
A14 tatABC_RBS O14 O15 (63.5) F4 1634 Amplification of tatA with RBS  ?  ? 08/07
A15 pFecA  ?  ?  ? F4  ?  ?  ?  ? 08/10



http://parts.mit.edu/r/parts/images/partbypart/icon_terminator.png http://parts.mit.edu/images/partbypart/icon_regulatory.png http://parts.mit.edu/r/parts/images/partbypart/icon_rbs.png http://parts.mit.edu/r/parts/images/partbypart/icon_reporter.png http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png http://parts.mit.edu/registry/images/2/2e/Part_icon_generator.png http://parts.mit.edu/registry/images/a/ab/Part_icon_plasmid.png

Retrieved from "http://2009.igem.org/Team:Paris/Freezer_PCR_products"