Team:BIOTEC Dresden/Methods Vesicles

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The microfluidic system consists of a flow chamber made of Polydimethylsiloxane (PDMS) and a pump system that controls the flow rates of the various liquids into the chamber. Droplets are created within a defined space in the chamber and are propagated along a grid that allows containment and imaging. Two types of chambers have been used, differing in the geometry of the space where droplets were produced. One featured T-junction, and the other a V-junction (Fig1).
The microfluidic system consists of a flow chamber made of Polydimethylsiloxane (PDMS) and a pump system that controls the flow rates of the various liquids into the chamber. Droplets are created within a defined space in the chamber and are propagated along a grid that allows containment and imaging. Two types of chambers have been used, differing in the geometry of the space where droplets were produced. One featured T-junction, and the other a V-junction (Fig1).
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Fig1: Flow chamber with T shaped junction between aqueous and lipid phases
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  {{multiple image
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[[Image:tchamber2.jpg|300px]]
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  | width    = 300
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[[Image:Amun.svg|thumb|upright=0.56|alt=Full-length profile of man in ancient Egyptian clothing. He has red-brown skin and wears a helmet with tall yellow plumes.|The Egyptian god Amun, portrayed before the Amarna period]]
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  | footer    = Different geometries at intersection of aqueous and lipid phases in flow chambers
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  | image1    = tchamber2.jpg
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  | alt1      = T junction
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  | caption1  = Caution
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  | image2    = Vchamber.jpg
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  | alt2      = V junction
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  | caption2  = Ejection
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  }}
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Production of flow chambers:
Production of flow chambers:

Revision as of 18:02, 19 October 2009

Vesicles - Methods

Setup of the microfluidic system

The microfluidic system consists of a flow chamber made of Polydimethylsiloxane (PDMS) and a pump system that controls the flow rates of the various liquids into the chamber. Droplets are created within a defined space in the chamber and are propagated along a grid that allows containment and imaging. Two types of chambers have been used, differing in the geometry of the space where droplets were produced. One featured T-junction, and the other a V-junction (Fig1). 

 Template loop detected: Template:Multiple image


Production of flow chambers:

  • mix PDMS and curing agent in 10:1 ratio
  • degas and pour on wafer with etched microstructures
  • polymerize on heat plate at 150 ºC for 30 min
  • add unpolymerized PDMS mixture to points on microstructure where microtube inlets are to be pierced
  • polymerize on heat plate at 150 ºC for 30 minutes
  • remove polymerized PDMS from wafer, cut to fit onto glass cover slide (24 x 60 mm), and use clean needles (0.8 mm) or laser cutter to pierce tube inlets
  • ionize PDMS and glass slide in plasma chamber for 30 sec to make it reactive
  • align PDMS on glass slide and seal
  • seal irreversibly by heating on plate at 60ºC for 6 hours


The pumping system (ceDOSYS SP-4) allows control of syringes filled with aqueous material and surfactant treated with mineral oil, respectively. The syringes access the chamber via the tubing inlets. Two inlets are used to pump in material in the aqueous phase; the remaining one is used for the oil phase. The flow rates of the syringes are controlled via a ceDOSYS user interface software.

Control via pump system:

  • two syringes are loaded with 1ml each of material in the aqueous phase; during the first trial, distilled water
  • another is filled with a 1ml solution of 0.5% span 80 in oil
  • use flow rate on ceDOSYS interface to flood the chamber first with oil phase
  • gradually introduce aqueous phase and modify rates of both phases until the shear stress breaks the aqueous phase into droplets at the T- or V- junctions in the respective chambers






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